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The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations.
- M. Collins, P. Lawson, J. Farrow
- BiologyInternational Journal of Systematic Bacteriology
- 1 October 1994
The 16S rRNA gene sequences were determined by PCR direct sequencing and were compared with more than 80 previously determined clostridial sequences and the previously published sequences of representative species of other low- G + C-content gram-positive genera, thereby providing an almost complete picture of the genealogical interrelationships of theClostridia.
Distribution of menaquinones in actinomycetes and corynebacteria.
- M. Collins, T. Pirouz, M. Goodfellow, D. Minnikin
- Biology, ChemistryJournal of General Microbiology
- 1 June 1977
Menaquinones were the only isoprenoid quinones found in 48 corynebacteria and actinomycete strains examined, and Actinomadura dassonvillei strains had a characteristic pattern of di-, tetra- and hexahydromenaquinONES with 10 isoprene units which was slightly different from the pattern in mixtures of similar quInones from Actinomyces israelii and Act inomyces viscosus.
The genome sequence of Schizosaccharomyces pombe
The genome of fission yeast (Schizosaccharomyces pombe), which contains the smallest number of protein-coding genes yet recorded for a eukaryote, is sequenced and highly conserved genes important for eukARYotic cell organization including those required for the cytoskeleton, compartmentation, cell-cycle control, proteolysis, protein phosphorylation and RNA splicing are identified.
Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication.
Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica
The authors' analysis indicates that B. parapertussis and B. pertussis are independent derivatives of B. bronchiseptica-like ancestors; host adaptation seems to be a consequence of loss, not gain, of function, and differences in virulence may be related to loss of regulatory or control functions.
Direct Analysis of Genes Encoding 16S rRNA from Complex Communities Reveals Many Novel Molecular Species within the Human Gut
The majority of generated rDNA sequences did not correspond to known organisms and clearly derived from hitherto unknown species within this human gut microflora, including Clostridium coccoides and Eubacterium rectale.
Isoprenoid quinone analyses in bacterial classification and identification
- M. Collins
Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test
Comparative 16S rRNA sequence analysis has demonstrated that the genusBacillus consists of at least five phyletic lines, and the genusPaenibacillus can be readily distinguished from otherBacilli groups using a battery of phenotypic characters and a highly specific gene probe based on 16SrRNA.
Genetic Analysis of the Capsular Biosynthetic Locus from All 90 Pneumococcal Serotypes
This work provides the sequences of the capsular biosynthetic genes of all 90 serotypes of Streptococcus pneumoniae and relate these to the known polysaccharide structures and patterns of immunological reactivity of typing sera, thereby providing the most complete understanding of the genetics and origins of bacterial poly Saccharide diversity.
Identification of Enterococcus species isolated from human infections by a conventional test scheme
Streptococci (206 cultures) previously identified as enterococci were retrieved from storage and reidentified by using tests designed to identify species of the genus Enterococcus by using DNA-DNA hybridizations to assure correct identification.