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Protein and peptide arrays: recent trends and new directions.
Digital Detection of Exosomes by Interferometric Imaging
This work presents a method based on Single Particle Interferometric Reflectance Imaging Sensor (SP-IRIS) that allows multiplexed phenotyping and digital counting of various populations of individual exosomes captured on a microarray-based solid phase chip and foresee revolutionary implications in the clinical field with improvements in diagnosis and stratification of patients affected by different disorders.
Quantification of DNA and protein adsorption by optical phase shift.
Membrane-binding peptides for extracellular vesicles on-chip analysis
- A. Gori, Alessandro Romanato, M. Cretich
- Biology, ChemistryJournal of Extracellular Vesicles
- 24 September 2019
The use of membrane-sensing peptides are introduced as new, highly efficient ligands to directly integrate sEV capturing and analysis on a microarray platform and it is anticipated that this new class of ligands may greatly enrich the molecular toolbox for EV analysis.
New adsorbed coatings for capillary electrophoresis
A fully automated coating procedure was devised based on the use of diluted water solutions of new acrylic polymers bearing oxirane groups, which rapidly adsorbed from water onto the capillary wall, thus suppressing electroosmotic flow to a negligible value.
Protein microarray technology: how far off is routine diagnostics?
This review article will focus on the following operational challenges that are crucial for the use of microarrays in clinical settings: probe printing and quality control, procurement of bio-reagents and antibody cross-reactivity, and mass transport limitations and assay automation.
Development of a high-sensitivity immunoassay for amyloid-beta 1-42 using a silicon microarray platform.
Integrated platform for detecting pathogenic DNA via magnetic tunneling junction-based biosensors
Detection of allergen specific immunoglobulins by microarrays coupled to microfluidics
An improved microarray coupled to microfluidics for the detection of allergen specific immunoglobulin E (IgE) and the binding of high affinity IgE antibodies is enhanced whereas that of low affinity IgG antibodies, which are present at higher concentration, is impaired.
Multispot, label-free biodetection at a phantom plastic–water interface
- F. Giavazzi, M. Salina, M. Buscaglia
- BiologyProceedings of the National Academy of Sciences
- 21 May 2013
This work proposes and demonstrates an extremely simple, low-cost, label-free biodetector based on measuring the intensity of light reflected by the interface between a fluid sample and an amorphous fluoropolymer substrate having a refractive index very close to that of water and hosting various antibodies immobilized in spots.