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Genome sequence of the button mushroom Agaricus bisporus reveals mechanisms governing adaptation to a humic-rich ecological niche
TLDR
Observations reveal genetic and enzymatic mechanisms governing adaptation to the humic-rich ecological niche formed during plant degradation, further defining the critical role such fungi contribute to soil structure and carbon sequestration in terrestrial ecosystems.
Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns.
TLDR
A "Molecular Toolkit" comprising interchangeable promoters and marker genes to facilitate transformation of homobasidiomycete mushrooms is developed and it is demonstrated that a prerequisite for GFP expression in C. cinereus and A. bisporus is the presence of an intron.
Double-stranded RNA elements associated with the MVX disease of Agaricus bisporus.
TLDR
On-farm observations suggest that MVX could be spread by infected spores and/or mycelial fragments, and previously, La France disease was effectively contained and controlled by minimising the on-farm production and spread of basidiospores.
A phylogenetic reconstruction and emendation of Agaricus section Duploannulatae
TLDR
DNA sequence data from the ITS segments of the nuclear ribosomal DNA region was used to identify characters shared by section Duploannulatae, to further delimit species-level taxa within the section, and to develop a phylogenetic hypothesis.
The Origin of Multiple B Mating Specificities in Coprinus cinereus
TLDR
The number of B specificities possible by combination of the alleles that are described is 70, close to previous estimates based on population analysis, indicating that complex evolutionary processes gave rise to the B loci.
A comparison of methods for successful triggering of gene silencing in Coprinus cinereus
TLDR
The GFP reporter gene was exploited to evaluate and quantify efficacy of three different silencing strategies, and it was revealed that the antisense and hairpin sequences yielded similar levels of GFP suppression, and were both more efficient than untranslatable sense sequences.
Analysis of cDNA transcripts from Coniothyrium minitans reveals a diverse array of genes involved in key processes during sclerotial mycoparasitism.
TLDR
This study identified 251 unisequences representing genes preferentially expressed by C. minitans during sclerotial mycoparasitism, substantially increasing the molecular knowledge of this commercially important biocontrol agent.
Use of Coniothyrium minitans as a biocontrol agent and some molecular aspects of sclerotial mycoparasitism
TLDR
A number of studies have examined production and application methods, integrated control, ecology, and modes of action in order to understand the biology of the mycoparasite and enhance activity and reproducibility of use.
Polypropylene straw ampoules for the storage of microorganisms in liquid nitrogen
Abstract Liquid nitrogen storage systems are widely used for long term preservation of a range of microorganisms. Strains are stored in ampoules immersed in nitrogen or held in its evolving vapour.
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