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Autofluorescence-Free Live-Cell Imaging Using Terbium Nanoparticles.
TLDR
The application of a new type of surface-photosensitized terbium NPs for autofluorescence-free intracellular imaging in live HeLa cells shows the unprecedented photophysical properties of Tb-NPs for live-cell imaging that extend well beyond currently available nanometric imaging agents.
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Role of Nerve Growth Factor in Development and Persistence of Experimental Pulmonary Hypertension.
TLDR
Ex vivo/in vitro, it is found that NGF promotes pulmonary vascular cell proliferation and migration, pulmonary arterial hyperreactivity, and secretion of proinflammatory cytokines, and in vivo it is demonstrated that anti-NGF blocking antibodies prevent and reverse PH in rats through significant reduction of pulmonary arterials inflammation, hyperre activity, and remodeling.
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Nanoscale characterization of vesicle adhesion by normalized total internal reflection fluorescence microscopy.
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Time-gated FRET nanoassemblies for rapid and sensitive intra- and extracellular fluorescence imaging
TLDR
Effective brightness enhancement by FRET from several Tb to the same QD, the use of low nanomolar concentrations, and the quick and sensitive detection void of FRET acceptor background fluorescence are important advantages for advanced intra- and extracellular imaging of biomolecular interactions.
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Lanthanide-Complex-Loaded Polymer Nanoparticles for Background-Free Single-Particle and Live-Cell Imaging
TLDR
The objective was to establish an experimental procedure and show direct AFMs of lanthanides and lanthanide complexes that unequivocally can be assigned as single molecules or nanoparticles with respect to the structure and function of cells and tissues.
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Single‐Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots
TLDR
Developing of quantum dots (QDs) with two different SiO2 shell thicknesses that are coated withTwo different lanthanide complexes that resulted in four distinct photoluminescence decays that were encoded by simple time‐gated PL intensity detection in three individual temporal detection windows are reported.
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Topography of Cells Revealed by Variable-Angle Total Internal Reflection Fluorescence Microscopy.
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Optimizing FRET on Aluminum Surfaces via Controlled Attachment of Fluorescent Dyes
Forster resonance energy transfer (FRET) between fluorescent dyes is a frequently applied technique for analyzing concentrations and conformations of biomolecules. Optimizing FRET by controlled…
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Incidence angle calibration for prismless total internal reflection fluorescence microscopy.
We propose a calibration routine useful to evaluate the incident angle in total internal reflection fluorescence (TIRF) microscopy. This procedure is based on critical angle measurements conducted in…
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Membrane-substrate separation distance assessed by normalized total internal reflection fluorescence microscopy
As a consequence of the recent progress in nanoscale technology, more and more sensitive methods are developed to characterize and understand the dynamic of cell membrane adhesion process. In this…
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