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Development of protocols for detection of Colletotrichum acutatum and monitoring of strawberry anthracnose using real‐time PCR
The new real-time PCR assays were shown to be useful tools for rapid detection and identification of these pathogens and to allow rapid and accurate assessment of the casual agents of anthracnose in strawberry.
Proteomic analysis of phytopathogenic fungus Botrytis cinerea as a potential tool for identifying pathogenicity factors, therapeutic targets and for basic research
- F. J. Fernández‐Acero, I. Jorge, J. Cantoral
- Biology, MedicineArchives of Microbiology
- 16 February 2007
The results indicate that proteomic analysis are becoming an important tool to be used as a starting point for identifying new pathogenicity factors, therapeutic targets and for basic research on this plant pathogen in the postgenomic era.
2‐DE proteomic approach to the Botrytis cinerea secretome induced with different carbon sources and plant‐based elicitors
The observed increases in secretion of proteins with established virulence‐related functions indicate that this in vitro‐induction/proteome‐mining approach is a promising strategy for discovering new pathogenicity factors and dissecting infection mechanisms in a discrete fashion.
Phylogenetic relationships and genome organisation of Colletotrichum acutatum causing anthracnose in strawberry
- C. Garrido, M. Carbú, Francisco Javier Fernández-Acero, I. Vallejo, J. Manuel Cantoral
- BiologyEuropean Journal of Plant Pathology
- 10 June 2009
The phylogenetic relationships within a worldwide sample of fifty-two C. acutatum isolates collected from different strawberry cultivars have been established, by using ITS sequence analyses and comparing karyotypes patterns and southern-blot analysis demonstrated a high level of molecular polymorphism.
Two‐dimensional electrophoresis protein profile of the phytopathogenic fungus Botrytis cinerea
A protocol for protein extraction from mycelium tissue, with protein separation by 2‐DE and MS analysis, was optimised as a first approach to defining the B. cinerea proteome.
Proteomic Advances in Phytopathogenic Fungi
Fungal proteomic is still at an early stage, but recent reports show that it is a potential tool for identifying pathogenicity factors, therapeutic targets and for basic research.
Isolation and pathogenicity of Colletotrichum spp. causing anthracnose of strawberry in south west Spain
Anthracnose, caused by Colletotrichum spp., is a major disease of cultivated strawberry, Fragaria × ananassa. This study identifies the Colletotrichum spp. which causes strawberry anthracnose in the…
Development of Proteomics-Based Fungicides: New Strategies for Environmentally Friendly Control of Fungal Plant Diseases
- Francisco Javier Fernandez Acero, M. Carbú, M. R. El-Akhal, C. Garrido, V. González-Rodríguez, J. Cantoral
- BiologyInternational journal of molecular sciences
- 21 January 2011
Previous research efforts are summarized and future innovations, focused on the fight against one of the main agents causing a devastating crops disease, fungal phytopathogens, are discussed.
The influence of yeast on chemical composition and sensory properties of dry white wines.