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The post-transcriptional steps of eukaryotic ribosome biogenesis
TLDR
Quality control mechanisms are being discovered that monitor ribosome synthesis and degrade the RNA components of defective pre-ribosomal particles.
The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation.
TLDR
Nine novel human ACA snoRNAs (U64-U72) are characterized, demonstrating that these RNAs possess closely related nucleolar functions and sharing a common secondary structure and conserved box elements that likely function as binding sites for common proteins.
The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase.
TLDR
It is concluded that Cbf5p is likely to be the rRNA Psi synthase and is an integral component of the box H + ACA class of snoRNPs, which function to target the enzyme to its site of action.
Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs
TLDR
The results suggest that Nhp2p and Nop10p, together with Cbf5p, constitute the core of H/ACA snoRNPs.
The ATPase and helicase activities of Prp43p are stimulated by the G‐patch protein Pfa1p during yeast ribosome biogenesis
TLDR
The results strongly suggest that stimulation of ATPase/helicase activities of Prp43p by Pfa1p is required for efficient 20S pre‐rRNA‐to‐18S rRNA conversion.
GAR1 is an essential small nucleolar RNP protein required for pre‐rRNA processing in yeast.
TLDR
Immunoprecipitation with anti‐GAR1 antibodies shows that GAR1 is associated with a subset of snoRNAs, including snR10 and snR30, and is involved in rRNA metabolism.
The Splicing ATPase Prp43p Is a Component of Multiple Preribosomal Particles
TLDR
It is found that depletion of Prp43p leads to accumulation of 35S pre-rRNA and strongly reduces levels of all downstream pre- rRNA processing intermediates, and the steady-state levels of mature rRNAs are greatly diminished following Prp 43p depletion.
Late Cytoplasmic Maturation of the Small Ribosomal Subunit Requires RIO Proteins in Saccharomyces cerevisiae
TLDR
The results strongly suggest that Rio2p and Rrp10p/Rio1p are shuttling proteins which associate with pre-40S particles in the nucleus and they are absolutely required for the cytoplasmic maturation of 20S pre-rRNA at site D, leading to mature 40S ribosomal subunits.
Rok1p is a putative RNA helicase required for rRNA processing
TLDR
Results suggest that Rok1p is required for the function of the snoRNP complex carrying out the early pre-rRNA cleavage reactions, and indirect immunofluorescence by using a ProtA-Rok 1p construct shows the protein to be predominantly nucleolar.
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