The Carnegie Protein Trap Library: A Versatile Tool for Drosophila Developmental Studies
- M. Buszczak, S. Paterno, A. Spradling
- BiologyGenetics
- 1 March 2007
Using transposon mutagenesis in Drosophila, a library of 7404 protein trap and enhancer trap lines, the Carnegie collection, is constructed to facilitate gene expression mapping at single-cell resolution and finds that 600–900 different genes are trapped in the collection.
Efficient protein trafficking requires trailer hitch, a component of a ribonucleoprotein complex localized to the ER in Drosophila.
- J. Wilhelm, M. Buszczak, Suzanne Sayles
- BiologyDevelopmental Cell
- 1 November 2005
The Drosophila melanogaster Cajal body
- Ji-Long Liu, C. Murphy, M. Buszczak, Sarah Clatterbuck, R. Goodman, J. Gall
- BiologyJournal of Cell Biology
- 13 March 2006
The identification of these nuclear bodies in D. melanogaster permits a broad range of questions to be asked about CB structure and function in a genetically tractable organism.
Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila.
- M. Buszczak, M. Freeman, J. Carlson, M. Bender, L. Cooley, W. Segraves
- BiologyDevelopment
- 15 October 1999
Data indicating that the ecdysone response hierarchy also mediates egg chamber maturation during mid-oogenesis and the temporal expression profile of E75, E74 and BR-C may be a functional reflection of ecDysone levels are suggested, suggesting that ecdYSone provides temporal signals regulating the progression of oogenesis and proper specification of dorsal follicle cell fates.
Changes in rRNA Transcription Influence Proliferation and Cell Fate Within a Stem Cell Lineage
- Qiao Zhang, Nevine A. Shalaby, M. Buszczak
- BiologyScience
- 17 January 2014
It is demonstrated that modulating rRNA synthesis fosters changes in the cell fate, growth, and proliferation of female Drosophila GSCs and their daughters.
Flytrap, a database documenting a GFP protein-trap insertion screen in Drosophila melanogaster
- R. Kelso, M. Buszczak, A. T. Quiñones, Claudia Castiblanco, S. Mazzalupo, L. Cooley
- BiologyNucleic Acids Res.
- 2004
Flytrap is a web-enabled relational database of transposable element insertions in Drosophila melanogaster that contains two principle data types: micrographs of protein localization and a cellular component ontology, based on rules derived from the Gene Ontology consortium.
Drosophila Stem Cells Share a Common Requirement for the Histone H2B Ubiquitin Protease Scrawny
- M. Buszczak, S. Paterno, A. Spradling
- BiologyScience
- 9 January 2009
This work describes a Drosophila gene, scrawny (scny), encoding a ubiquitin-specific protease, which is required in germline, epithelial, and intestinal stem cells and suggests it represents a common mechanism within stem cells that is used to repress the premature expression of key differentiation genes, including Notch target genes.
Mei-P26 regulates the maintenance of ovarian germline stem cells by promoting BMP signaling
- Yun Li, J. Maines, Omur Y. Tastan, D. Mckearin, M. Buszczak
- BiologyDevelopment
- 1 May 2012
Mei-P26 has distinct functions in the ovary and participates in regulating the fates of both GSCs and their differentiating daughters, suggesting that Mei-P 26 can enhance miRNA-mediated silencing in specific contexts.
Transforming activity of an oncoprotein-encoding circular RNA from human papillomavirus
- Jiawei Zhao, Eunice E. Lee, Richard C. Wang
- BiologyNature Communications
- 24 May 2019
Evidence is provided that virus-derived, protein-encoding circular RNAs are biologically functional and linked to the transforming properties of some HPV and show that circRNA-encoded E7 contributes to cancer cell growth in vitro and in tumor xenografts.
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