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New colorimetric cytotoxicity assay for anticancer-drug screening.
The SRB assay provides a sensitive measure of drug-induced cytotoxicity, is useful in quantitating clonogenicity, and is well suited to high-volume, automated drug screening.
Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.
Since the microculture tetrazolium assay provides sensitive and reproducible indices of growth as well as drug sensitivity in individual cell lines over the course of multiple passages and several months' cultivation, it appears suitable for initial-stage in vitro drug screening.
Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines.
The new XTT reagent provides for a simplified, in vitro cell growth assay with possible applicability to a variety of problems in cellular pharmacology and biology, but still shares many of the limitations and potential pitfalls of MTT or other tetrazolium-based assays.
Isolation and Characterization of Griffithsin, a Novel HIV-inactivating Protein, from the Red Alga Griffithsia sp.*
Data suggest that GRFT is a new type of lectin that binds to various viral glycoproteins in a monosaccharide-dependent manner and could be a potential candidate microbicide to prevent the sexual transmission of HIV and AIDS.
Discovery of cyanovirin-N, a novel human immunodeficiency virus-inactivating protein that binds viral surface envelope glycoprotein gp120: potential applications to microbicide development
The novel 11-kDa virucidal protein, named cyanovirin-N (CV-N), is isolated and sequenced from cultures of the cyanobacterium (blue-green alga) Nostoc ellipsosporum and is highly resistant to physicochemical denaturation, further enhancing its potential as an anti-HIV microbicide.
Display and analysis of patterns of differential activity of drugs against human tumor cell lines: development of mean graph and COMPARE algorithm.
This study developed a key methodological tool, the mean graph, which allowed the transformation of the numerical cell line response data into graphic patterns that were particularly expressive of differential cell growth inhibition.
Some practical considerations and applications of the national cancer institute in vitro anticancer drug discovery screen
Practical considerations and selected specific examples are selected, particularly illustrating research applications of the NCI screen that may be more broadly applicable to the search for new anticancer drug development leads with novel profiles of antitumor activity and/or mechanisms of action.
Tetrazolium-based assays for cellular viability: a critical examination of selected parameters affecting formazan production.
- D. Vistica, P. Skehan, D. Scudiero, A. Monks, A. Pittman, M. Boyd
- Biology, ChemistryCancer Research
- 15 May 1991
Results indicate that MTT specific activity is significantly influenced by a number of parameters and suggest that assay conditions should be established which minimize their effects.
New soluble-formazan assay for HIV-1 cytopathic effects: application to high-flux screening of synthetic and natural products for AIDS-antiviral activity.
- O. Weislow, R. Kiser, D. Fine, J. Bader, R. Shoemaker, M. Boyd
- BiologyJournal of the National Cancer Institute
- 19 April 1989
This simple microassay minimizes the number of plate manipulations typically required with other assay methods and, coupled with computerized data collection and analysis, facilitates large-scale screening of agents for potential antiviral activity.