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Conjugative transfer of clostridial shuttle vectors from Escherichia coli to Clostridium difficile through circumvention of the restriction barrier
Progress towards understanding the molecular basis of virulence in Clostridium difficile has been hindered by the lack of effective gene transfer systems. We have now, for the first time, developedExpand
Molecular Architecture and Functional Analysis of NetB, a Pore-forming Toxin from Clostridium perfringens*
TLDR
The crystal structure of the pore form of NetB is presented, showing that NetB does not bind phosphocholine efficiently but instead interacts directly with cholesterol leading to enhanced oligomerization and pore formation and providing potential new tools to the field of bionanotechnology. Expand
Twinkle helicase is essential for mtDNA maintenance and regulates mtDNA copy number.
TLDR
It is demonstrated for the first time that increased expression of Twinkle in muscle and heart increases mtDNA copy number up to 3-fold higher than controls, more than any other factor reported to date. Expand
Molecular basis of toxicity of Clostridium perfringens epsilon toxin
TLDR
The exquisite specificity of the toxin for specific cell types suggests that it binds to a receptor found only on these cells, and the crystal structure of ε‐toxin reveals similarity to aerolysin from Aeromonas’hydrophila, parasporin‐2 from Bacillus’thuringiensis and a lectin from Laetiporus’sulphureus. Expand
A Burkholderia pseudomallei Toxin Inhibits Helicase Activity of Translation Factor eIF4A
TLDR
The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. Expand
Cryo-EM structure of lysenin pore elucidates membrane insertion by an aerolysin family protein
TLDR
The pore structure of lysenin is determined by single particle cryo-EM to 3.1 Å resolution and reveals a long β-barrel channel spanning the length of the complex that, unexpectedly, includes the two pre-insertion strands flanking the hypothetical membrane- Insertion loop. Expand
Clostridium perfringens epsilon toxin H149A mutant as a platform for receptor binding studies
TLDR
The role of surface exposed tyrosine residues in domain I of E tx in binding to MDCK cells and the suitability of Etx‐H149A for further receptor binding studies are confirmed and have important implications for developing strategies designed to neutralise toxin activity. Expand
The conserved translocase Tim17 prevents mitochondrial DNA loss.
TLDR
Tim17 is a conserved suppressor of mtDNA instability and is the first factor to be identified that can prevent mtDNA loss in a human cellular model of mitochondrial disease. Expand
Expression of algal nuclear ATP synthase subunit 6 in human cells results in protein targeting to mitochondria but no assembly into ATP synthase.
TLDR
Nuclear compatible versions of human subunit a (A6) of ATP synthase, linked to a mitochondrial targeting signal are created, finding obstacles remain to functional expression of mitochondrial genes transferred to the nucleus. Expand
Clostridium perfringens epsilon toxin mutant Y30A-Y196A as a recombinant vaccine candidate against enterotoxemia
TLDR
Etx mutant Y30A-Y196A showed markedly reduced cytotoxicity towards MDCK and could form the basis of a recombinant vaccine against enterotoxemia. Expand
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