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Detecting substeps in the rotary motors of FoF1-ATP synthase by Hidden Markov Models
TLDR
The basic prerequisites for the experimental single-molecule FRET data are defined that allow for discrimination between a 120o stepping mode or a 36o substep rotation mode for the proton-driven Fo motor of ATP synthase.
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Targeting cytochrome C oxidase in mitochondria with Pt(II)-porphyrins for photodynamic therapy
TLDR
Electron microscopy revealed the disruption of the mitochondrial christae as a primary step of Photodynamic Therapy (PDT), and time resolved phosphorescence measurements identified COX as the binding site for Pt(II)-TMPyP in living HeLa cells.
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Poly(BODIPY)s: A New Class of Tunable Polymeric Dyes
We present a new class of polymeric dyes bearing the difluoroboraindacene (BODIPY) chromophore within the main chain. Starting from a diiodinated BODIPY monomer, homo- and copolymers with a fully c...
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Asymmetry of rotational catalysis of single membrane-bound F0F1-ATP synthase
TLDR
Computer simulations of the FRET signals and non-equally distributed orientations of ε strongly corroborate asymmetry of catalysis in F0F1-ATP synthase.
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Monitoring the conformational dynamics of a single potassium transporter by ALEX-FRET
TLDR
The study aims at the observation of conformational fluctuations within a single P-type ATPase functionally reconstituted into liposomes by single-molecule FRET and analysis by Hidden-Markov-Models.
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MITCHELL MEDAL LECTURE
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Subunit rotation in a single FoF1-ATP synthase in a living bacterium monitored by FRET
TLDR
Progress is reported of measuring subunit rotation in single FoF1-ATP synthases in vitro and in vivo, which was enabled by a new labeling approach for single-molecule FRET measurements.
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Monitoring single membrane protein dynamics in a liposome manipulated in solution by the ABELtrap
TLDR
A combination of the ABELtrap with confocal FRET measurements to monitor single membrane enzyme dynamics by FRET for more than 10 seconds in solution and evaluates two different methods to trap the enzyme inside the confocal volume in order to extend the observation times.
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Regulatory conformational changes of the epsilon subunit in single FRET-labeled FoF1-ATP synthase
TLDR
Labeling the C-terminal domain of epsilon and the gamma subunit specifically with two different fluorophores for single-molecule Foerster resonance energy transfer (smFRET) allowed monitoring of the conformation of ePSilon in the reconstituted enzyme in real time.
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Regulatory assembly of the vacuolar proton pump VoV1-ATPase in yeast cells by FLIM-FRET
TLDR
The reversible disassembly of VOV1-ATPase in life yeast cells by time resolved confocal FRET imaging is investigated by tagging two subunits C and E with different fluorescent proteins to follow the disassembly / reassembly in vivo.
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