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Structure of 20S proteasome from yeast at 2.4Å resolution
TLDR
Two β-type subunits are processed to an intermediate form, indicating that an additional nonspecific endopeptidase activity may exist which is important for peptide hydrolysis and for the generation of ligands for class I molecules of the major histocompatibility complex. Expand
The structures of HsIU and the ATP-dependent protease HsIU-HsIV.
TLDR
HslV and HslU display sixfold symmetry, ruling out mechanisms of protease activation that require a symmetry mismatch between the two components, and are the first structure of a complete set of components of an ATP-dependent protease. Expand
The structures of HslU and the ATP-dependent protease HslU–HslV
TLDR
The crystal structures of free HslU and an 820,000 relative molecular mass complex of HSlU and HslV are reported–the first structure of a complete set of components of an ATP-dependent protease. Expand
Mutational studies on HslU and its docking mode with HslV.
TLDR
ATP-binding site mutations confirm the essential role of the "sensor arginine" and the "arginine finger" in the ATPase action of HslU and demonstrate an important role for E321, and a better refined structure of the HslVU complex crystallized along with resorufin-labeled casein is reported. Expand
The catalytic sites of 20S proteasomes and their role in subunit maturation: a mutational and crystallographic study.
TLDR
A gallery of proteasome mutants that contain active site residues in the context of the inactive subunits beta3(Pup3), beta6(Pre7), and beta7(Pre4) show that the presence of Gly-1, Thr1, Asp17, Lys33, Ser129, AsP166, and Ser169 is not sufficient to generate activity. Expand
Molecular Machines for Protein Degradation
TLDR
The current state of knowledge about protein‐degradation systems is summarized, focusing in particular on the proteasome, HslVU, Tricorn protease and its interacting factors and DegP. Expand
Latent LytM at 1.3A resolution.
TLDR
It is shown that a truncated version of LytM that lacks the N-terminal part with the poorly conserved Zn(2+) ligand N117 has much higher specific activity than full-length enzyme. Expand
Crystal structures of active LytM.
TLDR
The synthesis of a convenient reporter substrate is described, the metal and pH-dependence of an active LytM fragment is characterized, and its crystal structure is presented in three crystal forms at different pH values that either support or do not support activity. Expand
Crystal structure of heat shock locus V (HslV) from Escherichia coli.
TLDR
The crystal structure of HslV at 3.8-A resolution, determined by isomorphous replacement and symmetry averaging, shows that in spite of the different symmetry of the particle, the fold and the contacts between subunits are conserved. Expand
DNA demethylation pathways: Additional players and regulators
TLDR
New data clarify the roles and the regulation of well‐known enzymes in active DNA demethylation, identify base excision repair (BER) glycosylases that may cooperate with or replace thymine DNA Glycosylase (TDG) in the base excison step, and suggest possible involvement of DNA damage repair pathways other than BER in activeDNA demethylations. Expand
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