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Subcellular Discharge of a Serine Protease Mediates Release of Invasive Malaria Parasites from Host Erythrocytes
A single fragment of a malaria merozoite surface protein remains on the parasite during red cell invasion and is the target of invasion- inhibiting antibodies
- M. Blackman, H. Heidrich, S. Donachie, J. McBride, A. Holder
- BiologyThe Journal of experimental medicine
- 1 July 1990
A complex of polypeptides derived from a precursor is present on the surface of the malaria merozoite and only a small fragment is retained on the parasite surface and carried into the newly infected red cell.
Molecular Identification of a Malaria Merozoite Surface Sheddase
Proteolytic shedding of surface proteins during invasion by apicomplexan parasites is a widespread phenomenon, thought to represent a mechanism by which the parasites disengage adhesin-receptor…
A Plant-Like Kinase in Plasmodium falciparum Regulates Parasite Egress from Erythrocytes
It is found that the plant-like calcium-dependent protein kinase PfCDPK5, which is expressed in invasive merozoite forms of Plasmodium falciparum, was critical for egress from the human host erythrocyte, an essential step in the parasite life cycle.
Intramembrane proteolysis mediates shedding of a key adhesin during erythrocyte invasion by the malaria parasite
A crucial role for intramembrane proteolysis in the life cycle of this pathogen is identified and mutations within the EBA-175 TMD that abolish cleavage by PfROM4 prevent parasite growth.
An Inhibitory Antibody Blocks Interactions between Components of the Malarial Invasion Machinery
It is shown that the invasion-inhibitory monoclonal antibody (mAb) 4G2, which recognises P. falciparum AMA1 (PfAMA1), cannot bind when PfAMA1 is in a complex with its partner proteins, and it is proposed that mAb 4G 2 inhibits invasion by preventing Pf AMA1 from interacting with other components of the invasion complex.
Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes
- R. Moon, Joanna Hall, M. Blackman
- Medicine, BiologyProceedings of the National Academy of Sciences
- 24 December 2012
Human-adapted P. knowlesi clones maintain their capacity to replicate in monkey erythrocytes and can be genetically modified with unprecedented efficiency, providing an important and unique model for studying conserved aspects of malarial biology as well as species-specific features of an emerging pathogen.
A Role for Apical Membrane Antigen 1 during Invasion of Hepatocytes by Plasmodium falciparum Sporozoites*
Investigation of the expression and function of another microneme protein recently identified in Plasmodium falciparum sporozoites, apical membrane antigen 1 (AMA-1), finds that interfering with sporozoite proteolytic processing may constitute a valuable strategy to prevent hepatocyte infection.
Malaria Parasite cGMP-dependent Protein Kinase Regulates Blood Stage Merozoite Secretory Organelle Discharge and Egress
The signalling pathway that regulates PfSUB1 function and egress is identified, and the possibility of targeting PfPKG or parasite phosphodiesterases in therapeutic approaches to dysregulate critical protease-mediated steps in the parasite life cycle is raised.
Naturally acquired cellular and humoral immune responses to the major merozoite surface antigen (Pf MSP1) of Plasmodium falciparum are associated with reduced malaria morbidity
Interestingly, proliferative responses to some regions of the molecule, including some highly conserved sequences, were highest in young children and decreased markedly with increasing age, and high concentrations of antibodies to a conserved region close to the N terminus of Pf MSP1 were also significantly associated with protection.