Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage
Using scRNA-seq analysis, Bhattacharya and colleagues identify a subset of profibrotic lung macrophages that have a gene expression signature intermediate between those of monocytes and alveolar macrophage.
Negative-Pressure Pulmonary Edema.
Impact of FDG PET on defining the extent of disease and on the treatment of patients with recurrent or metastatic breast cancer.
FDG PET contributes significantly to defining the extent of disease and deciding on treatment of patients with advanced breast cancer.
Absence of integrin αvβ3 enhances vascular leak in mice by inhibiting endothelial cortical actin formation.
- G. Su, A. Atakilit, D. Sheppard
- Biology, MedicineAmerican Journal of Respiratory and Critical Care…
- 14 December 2012
The studies identify a novel mechanism by which αvβ3 mitigates increased vascular leak, a pathophysiologic function central to sepsis and ALI.
Globular domains 4/5 of the laminin α3 chain mediate deposition of precursor laminin 5
It is concluded that the G4/5 domain in the α3 chain facilitates deposition of precursor laminin 5 into the PBM in epidermal wounds and was at least threefold more efficiently deposited than precleaved isoform.
IQGAP1 is necessary for pulmonary vascular barrier protection in murine acute lung injury and pneumonia.
- M. Bhattacharya, G. Su, D. Sheppard
- Biology, MedicineAmerican Journal of Physiology - Lung cellular…
- 1 July 2012
These experiments indicate that IQGAP1 is necessary for S1P-mediated vascular barrier protection during acute lung injury and is required for junctional localization of the barrier-protective integrin α(v)β(3).
Counter Regulation of Spic by NF-κB and STAT Signaling Controls Inflammation and Iron Metabolism in Macrophages
IQGAP1-dependent scaffold suppresses RhoA and inhibits airway smooth muscle contraction.
- M. Bhattacharya, A. Sundaram, D. Sheppard
- Biology, MedicineJournal of Clinical Investigation
- 3 November 2014
It is determined that IQGAP1 modulates airway smooth muscle contractility and the results suggest that the intracellular scaffold protein has the potential to modulate airway contraction severity in acute asthma.
Reference-based annotation of single cell transcriptomes identifies a profibrotic macrophage niche after tissue injury
The results uncover a specific pathologic subgroup of macrophages with markers that could enable their therapeutic targeting for fibrosis, and identify a macrophage subpopulation specific to injured lung and notable for high expression of Cx3cr1+ and MHCII genes.
Effective Treatment of Mouse Sepsis With an Inhibitory Antibody Targeting Integrin &agr;v&bgr;5*
It is suggested thatIntegrin &agr;v&bgr;5 is an important regulator of the vascular endothelial leak response in sepsis and that &agR;v &b gr;5 blockade may provide a novel approach to treating this devastating disease syndrome.