• Publications
  • Influence
Escherichia coli secretion of an active chimeric antibody fragment.
TLDR
The production in Escherichia coli of foreign heterodimeric protein reagents, such as Fab, should prove useful in the management of human disease. Expand
Isolation and characterization of the recA gene of Rhizobium meliloti
TLDR
Interspecific complementation of an Escherichia coli recA mutant with plasmids containing a gene bank of Rhizobium meliloti DNA was used to identify a clone which contains the recA gene of R.meliloti, and this bacterium was not deficient in its ability to carry out symbiotic nitrogen fixation. Expand
Structural relationships among rhizobium meliloti symbiotic promoters
TLDR
Examination of promoter and probable regulatory regions for these gene sets has revealed extensive DNA sequence conservation for more than 160 bp upstream of the respective transcription start points for three such promoter regions in the nitrogen fixation gene cluster of R. meliloti strain 102F34. Expand
Secretion of functional antibody and Fab fragment from yeast cells.
TLDR
Yeast strains that secrete functional mouse-human chimeric antibody and its Fab fragment into the culture medium are constructed and chimeric whole antibody exhibited antibody-dependent cellular cytot toxicity activity but not complement-dependent cytotoxicity activity. Expand
Potent anti-CD5 ricin A chain immunoconjugates from bacterially produced Fab' and F(ab')2.
TLDR
Genetic engineering was used to obtain secretion of anti-human CD5 antibody fragments from Escherichia coli for conjugation to the 30-kDa form of ricin A chain (RTA30) and these immunoconjugates efficiently killed a CD5+ T-cell line and human peripheral blood T cells. Expand
Cloning and expression of a gene encoding gelonin, a ribosome-inactivating protein from Gelonium multiflorum.
TLDR
Recombinant, soluble Gel (re-Gel) can be recovered from the E. coli culture supernatant at a yield of greater than 1 mg/ml, and it inhibits protein synthesis in vitro to the same extent as the native protein isolated from plant seeds. Expand
Preferential T-cell receptor beta-chain variable gene use in myelin basic protein-reactive T-cell clones from patients with multiple sclerosis.
TLDR
T cells specific for BP were cloned from the blood of multiple sclerosis patients and normal individuals, and expression of T-cell receptor variable region genes was analyzed, suggesting that the BP-specific clones derived from blood may be relevant to disease pathogenesis. Expand
Tumor burden, inflammation, and product attributes determine outcomes of axicabtagene ciloleucel in large B-cell lymphoma.
TLDR
Univariable and multivariable analyses indicated that rapid CAR T-cell expansion commensurate with pretreatment tumor burden, the number of CD8 and CCR7+CD45RA+ T cells infused, and host systemic inflammation, were the most significant determining factors for durable response. Expand
Human-engineered monoclonal antibodies retain full specific binding activity by preserving non-CDR complementarity-modulating residues.
TLDR
This model has been tested experimentally using H65, an anti-CD5 murine monoclonal antibody, whose binding activity had been greatly reduced by two previous attempts at humanization by conventional CDR grafting, and which retains the full binding avidity of parental murine and chimeric H65 antibodies. Expand
Axicabtagene ciloleucel, a first-in-class CAR T cell therapy for aggressive NHL
TLDR
Axicabtagene ciloleucel holds promise for the treatment of patients with CD19-positive malignancies, including refractory DLBCL. Expand
...
1
2
3
4
5
...