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Biochemistry, cell biology and molecular biology of lipids of Saccharomyces cerevisiae
The yeast Saccharomyces cerevisiae is a powerful experimental system to study biochemical, cell biological and molecular biological aspects of lipid synthesis. Most but not all genes encoding enzymesExpand
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Sterol Esterification in Yeast: A Two-Gene Process
Unesterified sterol modulates the function of eukaryotic membranes. In human cells, sterol is esterified to a storage form by acyl-coenzyme A (CoA): cholesterol acyl transferase (ACAT). Here, twoExpand
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Identification of a novel family of nonclassic yeast phosphatidylinositol transfer proteins whose function modulates phospholipase D activity and Sec14p-independent cell growth.
Yeast phosphatidylinositol transfer protein (Sec14p) is essential for Golgi function and cell viability. We now report a characterization of five yeast SFH (Sec Fourteen Homologue) proteins thatExpand
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Mutations in Yeast ARV1 Alter Intracellular Sterol Distribution and Are Complemented by Human ARV1 *
Intracellular cholesterol redistribution between membranes and its subsequent esterification are critical aspects of lipid homeostasis that prevent free sterol toxicity. To identify genes thatExpand
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Transcription of the Yeast Iron Regulon Does Not Respond Directly to Iron but Rather to Iron-Sulfur Cluster Biosynthesis*
Saccharomyces cerevisiae responds to iron deprivation by increased transcription of the iron regulon, including the high affinity cell-surface transport system encoded by FET3 and FTR1. Here weExpand
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Transcriptional regulation of the squalene synthase gene (ERG9) in the yeast Saccharomyces cerevisiae.
The ergosterol biosynthetic pathway is a specific branch of the mevalonate pathway. Since the cells requirement for sterols is greater than for isoprenoids, sterol biosynthesis must be regulatedExpand
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Positive and negative regulation of a sterol biosynthetic gene (ERG3) in the post‐squalene portion of the yeast ergosterol pathway
Regulation of sterol biosynthesis in the terminal portion of the pathway represents an efficient mechanism by which the cell can control the production of sterol without disturbing the production ofExpand
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Genome-wide expression profiling reveals genes associated with amphotericin B and fluconazole resistance in experimentally induced antifungal resistant isolates of Candida albicans.
OBJECTIVES The aim of this study was to identify changes in the gene expression profile of Candida albicans associated with the acquisition of experimentally induced resistance to amphotericin B andExpand
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Sterol synthesis and viability oferg11 (cytochrome P450 lanosterol demethylase) mutations inSaccharomyces cerevisiae andCandida albicans
The identification of the precise structural features of yeast sterol molecules required for the essential “sparking” function has been a controversial area of research. Recent cloning and geneExpand
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Transformation of Saccharomyces cerevisiae with a cDNA encoding a sterol C‐methyltransferase from Arabidopsis thaliana results in the synthesis of 24‐ethyl sterols
Using an EST‐cDNA probe, a full‐length cDNA (411) sequence of 1411 bp was isolated from A. thaliana. This sequence contained features typical of methyltransferases in general and in particular showedExpand
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