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Conversion of alpha-helices into beta-sheets features in the formation of the scrapie prion proteins.
  • K. Pan, M. Baldwin, +7 authors F. Cohen
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences…
  • 1 December 1993
Prions are composed largely, if not entirely, of prion protein (PrPSc in the case of scrapie). Although the formation of PrPSc from the cellular prion protein (PrPC) is a post-translational process,Expand
Binding of neural cell adhesion molecules (N-CAMs) to the cellular prion protein.
To identify molecular interaction partners of the cellular prion protein (PrP(C)), we sought to apply an in situ crosslinking method that maintains the microenvironment of PrP(C). Mild formaldehydeExpand
The characteristics of peptide collision-induced dissociation using a high-performance MALDI-TOF/TOF tandem mass spectrometer.
A new matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) high-resolution tandem mass spectrometer is described for sequencing peptides. This instrumentExpand
Structural studies of the scrapie prion protein using mass spectrometry and amino acid sequencing.
The only component of the infectious scrapie prion identified to date is a protein designated PrPSc. A posttranslational process converts the cellular PrP isoform (PrPC) into PrPSc. Denatured PrPScExpand
Pathway Complexity of Prion Protein Assembly into Amyloid*
In vivo under pathological conditions, the normal cellular form of the prion protein, PrPC (residues 23–231), misfolds to the pathogenic isoform PrPSc, a β-rich aggregated pathogenic multimer.Expand
Glycosylinositol phospholipid anchors of the scrapie and cellular prion proteins contain sialic acid.
The only identified component of the scrapie prion is PrPSc, a glycosylinositol phospholipid (GPI)-linked protein that is derived from the cellular isoform (PrPC) by an as yet unknownExpand
Identification of glycoinositol phospholipid linked and truncated forms of the scrapie prion protein.
Analysis of carboxy-terminal peptides derived from endoproteinase Lys-C digests of the scrapie isoform of the hamster prion protein revealed that the majority of the molecules are glycoinositolExpand
Mass Spectrometric Analysis of Protein Mixtures at Low Levels Using Cleavable 13C-Isotope-coded Affinity Tag and Multidimensional Chromatography*
In order to identify and compare the protein content of very low quantity samples of high complexity, a protocol has been established that combines the differential profiling strength of a newExpand
The Need for Guidelines in Publication of Peptide and Protein Identification Data
In an effort to ensure that high-quality, significant data are entering the proteomics literature, Molecular & Cellular Proteomics (MCP) is introducing guidelines for authors planning to submit manuscripts containing large numbers of proteins identified primarily by LC-MS/MS. Expand