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The medicinal leech genome encodes 21 innexin genes: different combinations are expressed by identified central neurons
Gap junctional proteins are important components of signaling pathways required for the development and ongoing functions of all animal tissues, particularly the nervous system, where they functionExpand
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Possible role of the receptor protein tyrosine phosphatase HmLAR2 in interbranch repulsion in a leech embryonic cell.
Accumulating evidence indicates that receptor protein tyrosine phosphatases (rPTPs) play major roles in growth cone migration. We have previously shown that the growth cones of the multiple parallelExpand
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Expression of a dominant negative mutant innexin in identified neurons and glial cells reveals selective interactions among gap junctional proteins
Neurons and glia of the medicinal leech CNS express different subsets of the 21 innexin genes encoded in its genome. We report here that the punctal distributions of fluorescently tagged innexinExpand
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The leech receptor protein tyrosine phosphatase HmLAR2 is concentrated in growth cones and is involved in process outgrowth.
Developing neurons extend long processes to specific distal targets using extracellular molecules as guidance cues to navigate through the embryo. Growth cones, specialized structures at the tip ofExpand
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Serotonin depletors, 5,7-dihydroxytryptamine and p-chlorophenylalanine, cause sprouting in the CNS of the adult snail
The effects of 5,7-dihydroxytryptamine (5,7-DHT) and p-chlorophenylalanine (PCPA) on neuronal morphology were investigated in Achatina fulica by backfilling the cerebrobuccal connective withExpand
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Microtargeted Gene Silencing and Ectopic Expression in Live Embryos Using Biolistic Delivery with a Pneumatic Capillary Gun
Modulating gene expression, either by transfection or by RNA-interference (RNAi), is a powerful means for studying the functions of essential genes during nervous system development and maintenance.Expand
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In vivo imaging of growth cone and filopodial dynamics: Evidence for contact‐mediated retraction of filopodia leading to the tiling of sibling processes
In the leech embryo, the peripheral comb cell (CC) sends out many nonoverlapping, growth cone‐tipped processes that grow in parallel and serve as a scaffold for the migrating myocytes of theExpand
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In vivo dynamics of CNS sensory arbor formation: a time-lapse study in the embryonic leech.
In the embryo of the leech Hirudo medicinalis, afferent projections of peripheral sensory neurons travel along common nerve tracts to the CNS, where they defasciculate, branch, and arborize intoExpand
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Serotonin depletion after prolonged chlorpromazine treatment in a simpler model system.
1. Prolonged exposure of the pond snail Lymnaea stagnalis to micromolar concentrations of chlorpromazine (CPZ) results in marked changes in the serotonin (5-HT) content of the central nervous system.Expand
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RNAi of the receptor tyrosine phosphatase HmLAR2 in a single cell of an intact leech embryo leads to growth-cone collapse
Receptor protein tyrosine phosphatases (RPTPs) are important for growth-cone migration [1-5], but their specific roles have yet to be defined. Previously, we showed that the growth cones of the CombExpand
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