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Trypanosomes lacking trypanothione reductase are avirulent and show increased sensitivity to oxidative stress
TLDR
The infectivity and virulence of the parasites in mice was dependent upon tetracycline‐regulated TRYR activity: if the trypanosomes were injected into mice in the absence of tetrACYcline, no infection was detectable; and when tetr Tracycline was withdrawn from previously infected animals, the parasitaemia was suppressed. Expand
Ovothiol and trypanothione as antioxidants in trypanosomatids.
TLDR
Under physiological conditions, ovothiol is unlikely to play a major role in the metabolism of hydrogen peroxide in intact cells, but this metabolite may have other important functional roles in trypanosomatids that could be exploited as a chemotherapeutic target. Expand
A Single Enzyme Catalyses Formation of Trypanothione from Glutathione and Spermidine in Trypanosoma cruzi *
TLDR
Kinetic studies on recombinant TcTryS purified from Escherichia coli revealed that the enzyme displays high-substrate inhibition with glutathione, but obeys Michaelis-Menten kinetics with spermidine, aminopropylcadaverine, glutathionylsperMidine, and MgATP as variable substrate. Expand
Diamine auxotrophy may be a universal feature of Trypanosoma cruzi epimastigotes.
TLDR
Results were obtained for epimastigotes representing all the major zymodeme classes, providing evidence that diamine auxotrophy may be a universal feature of this stage of the life-cycle. Expand
Phenotypic analysis of trypanothione synthetase knockdown in the African trypanosome.
TLDR
Depleted trypanothione levels were associated with increases in sensitivity to arsenical, antimonial and nitro drugs, implicating trypanothsione metabolism in their mode of action. Expand
Ornithine Decarboxylase Gene Deletion Mutants of Leishmania donovani *
TLDR
The data establish genetically that ODC is an essential gene in L. donovani, define the polyamine requirements of the parasite, and reveal the absence of a polyamine-degradative pathway. Expand
Properties of trypanothione synthetase from Trypanosoma brucei.
TLDR
The biosynthesis and degradation of trypanothione are similar in African and Americantrypanosomes, and different from the insect trypanosomatid, C. fasciculata and T. cruzi TRYS. Expand
Glutathionylspermidine metabolism in Escherichia coli.
TLDR
The much higher catalytic constant for the mixed disulphide of glutathione and glutathionylspermidine (11% that of GSSG), suggests a possible explanation for the low turnover of trypanothione disulPHide by E. coli. Expand
Characterization of recombinant glutathionylspermidine synthetase/amidase from Crithidia fasciculata.
TLDR
The successful expression of soluble (His)6-tagged C. fasciculata GspS in E. coli was reported, with a single amino acid mutation shown to ablate the amidase activity without affecting the synthetase activity. Expand
Activity of Megazol, a Trypanocidal Nitroimidazole, Is Associated with DNA Damage
TLDR
DNA damage associated with the trypanocidal activity of megazol was shown in experiments in which DNA repair-deficient RAD51−/−Trypanosoma brucei mutants were found to be hypersensitive to the drug. Expand
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