A microwell cytotoxicity assay using Artemia salina (brine shrimp).
- P. Solis, C. Wright, M. Anderson, M. Gupta, J. Phillipson
- BiologyPlanta Medica
- 1 June 1993
A new microplate assay for cytotoxicity testing using A. salina has been developed and shown to give results comparable to a previously published test-tube method, except for two which require metabolic activation in man.
Human neutrophils employ the myeloperoxidase-hydrogen peroxide-chloride system to convert hydroxy-amino acids into glycolaldehyde, 2-hydroxypropanal, and acrolein. A mechanism for the generation of…
- M. Anderson, S. L. Hazen, F. Hsu, J. Heinecke
- Chemistry, BiologyJournal of Clinical Investigation
- 1 February 1997
It is demonstrated that human neutrophils employ the myeloperoxidase-H202-chloride system of phagocytes to produce alpha-hydroxy and alpha,beta-unsaturated aldehydes from hydroxy-amino acids in high yield.
Identification of a cellular cofactor required for infection by feline leukemia virus.
- M. Anderson, A. Lauring, C. Burns, J. Overbaugh
- 10 March 2000
It is shown that an immunodeficiency-inducing, T cell-tropic feline leukemia virus (FeLV) has evolved such that it cannot infect cells unless both a classic multiple membrane-spanning receptor molecule (Pit1) and a second coreceptor or entry factor are present.
The myeloperoxidase system of human phagocytes generates Nepsilon-(carboxymethyl)lysine on proteins: a mechanism for producing advanced glycation end products at sites of inflammation.
- M. Anderson, J. Requena, J. Crowley, S. Thorpe, J. Heinecke
- Biology, ChemistryJournal of Clinical Investigation
- 1 July 1999
It is demonstrated that a model protein, ribonuclease A (RNase A), exposed to free L-serine and HOCl exhibits the biochemical hallmarks of advanced glycation end (AGE) products -- browning, increased fluorescence, and cross-linking.
Cloning of the cellular receptor for feline leukemia virus subgroup C (FeLV-C), a retrovirus that induces red cell aplasia.
- J. Quigley, C. Burns, J. Abkowitz
- 1 February 2000
It is hypothesized that the receptor cDNA of FeLV-C could have an essential role in early erythropoiesis, and a 6-kb fragment of the human FLVCR gene was amplified by polymerase chain reaction from genomic DNA, using homologous cDNA sequences identified in the human Expressed Sequence Tags database.
Molecular basis of human mitochondrial very-long-chain acyl-CoA dehydrogenase deficiency causing cardiomyopathy and sudden death in childhood.
- A. Strauss, C. K. Powell, H. Sims
- Biology, MedicineProceedings of the National Academy of Sciences…
- 7 November 1995
An initial delineation of human mutations in VLCAD suggests that V LCAD deficiency reduces myocardial fatty acid beta-oxidation and energy production and is associated with cardiomyopathy and sudden death in childhood.
Increased atherosclerosis in myeloperoxidase-deficient mice.
- M. Brennan, M. Anderson, A. Lusis
- BiologyJournal of Clinical Investigation
- 15 February 2001
It is shown here that neutrophils from homozygous mutants lack peroxidase and chlorination activity in vitro and fail to generate chlorotyrosine or to kill Candida albicans in vivo, suggesting an unexpected, protective role for MPO-generated reactive intermediates in murine atherosclerosis.
NMR lipid profiles of cells, tissues, and body fluids: proton NMR analysis of human erythrocyte lipids.
- R. Adosraku, G. Choi, V. Constantinou-Kokotos, M. Anderson, W. Gibbons
- Biology, PhysicsJournal of Lipid Research
- 1 November 1994
One- and two-dimensional high resolution NMR spectroscopy was applied to determine quantitatively and qualitatively the lipids extracted from human erythrocyte membranes and the results were compared to those obtained elsewhere using classical lipid analytical techniques and found to be in substantial agreement.
In vitro antiplasmodial, antiamoebic, and cytotoxic activities of a series of bisbenzylisoquinoline alkaloids
- S. Marshall, P. F. Russell, P. Schiff
- Chemistry, BiologyAntimicrobial Agents and Chemotherapy
- 1 January 1994
None of the 24 bisbenzylisoquinoline alkaloids exhibited significant cytotoxicity against the KB cell line, the most toxic being berbamine, with an IC50 of 17.8 microM (theIC50 of podophyllotoxin was 0.008 microM).
Human neutrophils employ the myeloperoxidase-hydrogen peroxide-chloride system to oxidize alpha-amino acids to a family of reactive aldehydes. Mechanistic studies identifying labile intermediates…
- S. L. Hazen, A. D'avignon, M. Anderson, F. Hsu, J. Heinecke
- Chemistry, BiologyJournal of Biological Chemistry
- 27 February 1998
The chemical mechanism and reaction intermediates generated during conversion of amino acids into aldehydes by myeloperoxidase-generated HOCl are detailed.