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Membrane Lipid Nanodomains.
This review summarizes the knowledge on lipid nanodomains in model membranes and exposes how their specific character contrasts with large-scale phase separation and the effects of curvature, asymmetry, and ions on membrane nanadomains are shown to be highly relevant aspects that may also modulate lipid nanadoms in cellular membranes.
Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane
Steady-state spectra as well as time-resolved emission spectra of the probes in solvents and model membranes revealed that they probe different properties of the lipid membrane, important for the application of these dyes in cell biology.
GM1 Ganglioside Inhibits β‐Amyloid Oligomerization Induced by Sphingomyelin
- M. Amaro, Radek Šachl, Gokcan Aydogan, I. Mikhalyov, Robert Vácha, M. Hof
- Biology, ChemistryAngewandte Chemie
- 13 June 2016
It is shown that sphingomyelin triggers oligomersization of Aβ40 and that GM1 is counteractive thus preventing oligomerization, suggesting that decreasing levels of GM1 in the brain, for example, due to aging, could reduce protection against Aβ oligomers and contribute to the onset of Alzheimer's disease.
Fluorescence of nitrobenzoxadiazole (NBD)-labeled lipids in model membranes is connected not to lipid mobility but to probe location.
- M. Amaro, H. Filipe, J. P. Prates Ramalho, M. Hof, Luís M. S. Loura
- ChemistryPhysical chemistry chemical physics : PCCP
- 2 March 2016
Nitrobenzoxadiazole (NBD)-labeled lipids are popular fluorescent membrane probes. However, the understanding of important aspects of the photophysics of NBD remains incomplete, including the observed…
Highly synergistic antimicrobial activity of magainin 2 and PGLa peptides is rooted in the formation of supramolecular complexes with lipids
- C. Aisenbrey, M. Amaro, P. Pospíšil, M. Hof, B. Bechinger
- Biology, ChemistryScientific Reports
- 15 July 2020
It is shown for the first time that the peptides assemble into defined supramolecular structures along the membrane interface, which explains their synergistic antimicrobial activity and provides a new concept to increase the therapeutic window of combinatorial drugs.
(GM1) Ganglioside Inhibits beta-Amyloid Oligomerization Induced bySphingomyelin
It is demonstrated that physiological levels of GM(1), organized in nanodomains do not seed oligomerization of A beta(40) monomers and thatGM(1) is counteractive thus preventing oligomership, and proposed a molecular explanation that is supported by all-atom molecular dynamics simulations.
Time-resolved fluorescence in lipid bilayers: selected applications and advantages over steady state.
On multivalent receptor activity of GM1 in cholesterol containing membranes.
Impact of GM1 on Membrane-Mediated Aggregation/Oligomerization of β-Amyloid: Unifying View.
Expansion of Access Tunnels and Active‐Site Cavities Influence Activity of Haloalkane Dehalogenases in Organic Cosolvents
- V. Štěpánková, Morteza Khabiri, J. Damborský
- Chemistry, BiologyChembiochem : a European journal of chemical…
- 10 May 2013
The use of enzymes for biocatalysis can be significantly enhanced by using organic cosolvents in the reaction mixtures. Selection of the cosolvent type and concentration range for an enzymatic…