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Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane
Analysis of frequently used polarity sensitive probes, Laurdan and di-4-ANEPPDHQ, to test whether the underlying physical mechanisms of their spectral changes are the same and whether they report on the same physico-chemical properties of the cell membrane. Expand
Fluorescence of nitrobenzoxadiazole (NBD)-labeled lipids in model membranes is connected not to lipid mobility but to probe location.
Nitrobenzoxadiazole (NBD)-labeled lipids are popular fluorescent membrane probes. However, the understanding of important aspects of the photophysics of NBD remains incomplete, including the observedExpand
Site-specific analysis of protein hydration based on unnatural amino acid fluorescence.
A simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy is described. Expand
(GM1) Ganglioside Inhibits beta-Amyloid Oligomerization Induced bySphingomyelin
beta-Amyloid (A beta) oligomers are neurotoxic and implicated in Alzheimer's disease. Neuronal plasma membranes may mediate formation of A beta oligomers in vivo. Membrane components sphingomyelinExpand
Membrane Lipid Nanodomains.
This review summarizes the knowledge on lipid nanodomains in model membranes and exposes how their specific character contrasts with large-scale phase separation and the effects of curvature, asymmetry, and ions on membrane nanadomains are shown to be highly relevant aspects that may also modulate lipid nanadoms in cellular membranes. Expand
GM1 Ganglioside Inhibits β‐Amyloid Oligomerization Induced by Sphingomyelin
It is shown that sphingomyelin triggers oligomersization of Aβ40 and that GM1 is counteractive thus preventing oligomerization, suggesting that decreasing levels of GM1 in the brain, for example, due to aging, could reduce protection against Aβ oligomers and contribute to the onset of Alzheimer's disease. Expand
Time-resolved fluorescence in lipid bilayers: selected applications and advantages over steady state.
Fluorescence methods are versatile tools for obtaining dynamic and topological information about biomembranes because the molecular interactions taking place in lipid membranes frequently occur onExpand
Comprehensive portrait of cholesterol containing oxidized membrane.
This contribution utilizing dual-focus fluorescence correlation spectroscopy and electron paramagnetic resonance as well as atomistic molecular dynamics simulations focuses on the overall changes of the membrane structural and dynamical properties once it becomes oxidized. Expand
On multivalent receptor activity of GM1 in cholesterol containing membranes.
The results indicate that membrane properties have an impact on a fraction of GM1 molecules that is not available for the ligand binding, while at low GM1 densities it is the cholesterol that turns GM1 headgroups invisible, at higher GM1 level it is purely the local density of GM 1 molecules that inhibits the recognition. Expand
Expansion of Access Tunnels and Active‐Site Cavities Influence Activity of Haloalkane Dehalogenases in Organic Cosolvents
The use of enzymes for biocatalysis can be significantly enhanced by using organic cosolvents in the reaction mixtures. Selection of the cosolvent type and concentration range for an enzymaticExpand