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Low temperature, IBA concentrations and optimal time for adventitious rooting of Eucalyptus benthamii mini-cuttings
The adventitious rooting percentage under different storage times in low temperatures and at various IBA (indole-3-butyric acid) concentrations was assessed to determine the optimal time of permanence for rooting Eucalyptus benthamii minicuttings in a greenhouse. Expand
Anatomical and biochemical characterization of the calcium effect on Eucalyptus urophylla callus morphogenesis in vitro
An increase in calcium concentration gave higher total protein and sugar contents, an increase in peroxidase specific activity and changes in the histological characteristics, and a multivariate statistical analysis grouped the treatments into 5 blocks and indicated the most responsive group. Expand
Micropropagation of Eucalyptus benthamii to form a clonal micro-garden
A method for cloning selected genotypes of E. benthamii using a micropropagation technique, enabling the formation of a clonal micro-garden, and normal development of adventitious roots showing a vascular connection with the vascular cambium is shown. Expand
Endophytic bacteria in long-term in vitro cultivated “axenic” pineapple microplants revealed by PCR–DGGE
This study is the first report that brings together evidences that pineapple microplants, previously considered axenic, harbor an endophytic bacterial community encompassing members of Actinobacteria, Alphaproteobacteria and BetaproteOBacteria group which is responsive to differences in organs due to plant development. Expand
Bacteriosomes in axenic plants: endophytes as stable endosymbionts
The existence of bacterial rRNA within axenic peach palm microplants raises the question of whether these stable endosymbionts were acquired in the process of evolution and how could they benefit theprocess of plants micropropagation. Expand
Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots
Histological analyses suggest that callus regeneration areas in hypocotyl explants recreate an appropriate niche for the development of pluripotent cells that give rise to buds and shoots in E. dunnii. Expand
Anatomical analysis of peach palm (Bactris gasipaes) leaves cultivated in vitro, ex vitro and in vivo
The results indicated that there were no discernible alterations of the anatomical characteristics of the leaves of micro-plants cultivated under differing conditions and that the thickening of the mesophyll and the vascular fibers indicated adaptive responses to ex vitro conditions, and the observed difficulties in acclimatizing peach palm micro-PLants to ex vivo conditions cannot be attributed to plant anatomical characteristics acquired during in vitro cultivation. Expand
Variability in essential oil composition produced by micropropagated (in vitro), acclimated (ex vitro) and in-field plants of Ocimum basilicum (Lamiaceae)
Abstract An in vitro protocol was developed for propagation of Ocimum basilicum from shoot cultures. Thus shoot apexes of the adult plant were used as explants and cultured and after eight monthsExpand
Interspecific xenia and metaxenia in seeds and fruits of tomato
Xenia, the transmission of traits from the pollinizer to the female's tissues, is a phenomenon hitherto unknown in tomatoes. Here, we describe xenia effects on the seeds and fruits of SolanumExpand
In vitro morphogenic response of leaf sheath of Phyllostachys bambusoides
Results show that Picloram induced the callogenesis in leaf sheath and the secondary embryogenesis was formed in yellow-globular callus, and sucrose as carbohydrate source in the absence of light was more efficient to induce rhizogenesis. Expand