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Phosphorylation of Hsl1 by Hog1 leads to a G2 arrest essential for cell survival at high osmolarity
A novel mechanism that regulates the Hsl1–Hsl7 complex to integrate stress signals to mediate cell cycle arrest is presented and it is demonstrated that a single MAPK coordinately modulates different cell cycle checkpoints to improve cell survival upon stress. Expand
Biophysical properties of Saccharomyces cerevisiae and their relationship with HOG pathway activation
Parameterized models of biophysical and mechanical cell properties are important for predictive mathematical modeling of cellular processes. The concepts of turgor, cell wall elasticity, osmoticallyExpand
Sic1 plays a role in timing and oscillatory behaviour of B-type cyclins.
This analysis suggests that the wave-like cyclins pattern derives from the binding of Sic1 to all Clb pairs rather than from Clb degradation, which highlights an additional role for Sic1 in regulating Cdk1-Clb complexes, coordinating their appearance. Expand
Prion Fibrillization Is Mediated by a Native Structural Element That Comprises Helices H2 and H3*
A “banana-peeling” mechanism for misfolding of PrP in which H2H3 is the aggregation seed that needs to be first exposed to promote conversion from a helical to a β-rich structure is suggested. Expand
Time-Dependent Quantitative Multicomponent Control of the G1-S Network by the Stress-Activated Protein Kinase Hog1 upon Osmostress
Different temporal roles are defined for the actions of Hog1 on Sic1 and cyclins in mediating G1 arrest upon hyperosmotic stress, which is essential for cell adaptation to extracellular stimuli. Expand
Mechanistic insights into cellular alteration of prion by poly‐D‐lysine: the role of H2H3 domain
It is shown that poly‐d‐lysine removes proteinase K‐resistant PrP from prion‐infected SN56 neuroblastoma cells without affecting PrPC, and a direct interaction between polylysine and PrP is confirmed, which mainly maps to the PrP region containing helices 2 and 3 (H2H3). Expand
Understanding cold denaturation: the case study of Yfh1.
By achieving nearly full assignment of the NMR spectrum, it is shown that at -1 °C, Yfh1 has all the features of an unfolded protein, although retaining some local, residual secondary structure. Expand
The role of zinc in the stability of the marginally stable IscU scaffold protein
It is demonstrated that the effect of zinc and its consequence strongly depend on the sample history, and how zinc coordination can be achieved by different side chains spatially available and all competent for tetrahedral coordination is discussed. Expand
Inhibition of Glycosylation Processes: the Reaction between Pyridoxamine and Glucose
A kinetic study of the reaction between glucose and pyridoxamine – a potent inhibitor of AGEs formation both in vivo and in vitro – was conducted, finding the NH2 group of pyrdoxamine was found to react with the CO group of glucose to form the Schiff base 9 (Scheme 2). Expand
Mechanistic insights in glycation-induced protein aggregation.
The molecular basis of how glycation is linked to aggregation is described by applying a variety of complementary techniques to study the nonenzymatic glycation of hen lysozyme with ribose (ribosylation) as the reducing carbohydrate. Expand