• Publications
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Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS)
The SAXS pipeline combines automated sample handling of microliter volumes, temperature and anaerobic control, rapid data collection and data analysis, and couples structural analysis with automated archiving to create an efficient pipeline enabling high-throughput analysis of protein structure in solution with small angle X-ray scattering. Expand
High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus.
These studies demonstrate that the error rate per nucleotide induced in the 182 known detectable sites of the lacI gene was 1.6 x 10(-6) for Pfu DNA polymerase, a greater than tenfold improvement over the 2.0 x 10 (-5) error rate for Taq DNA polymerases. Expand
The Iron-Hydrogenase of Thermotoga maritima Utilizes Ferredoxin and NADH Synergistically: a New Perspective on Anaerobic Hydrogen Production
The discovery of the bifurcating hydrogenase gives a new perspective on the understanding of the bioenergetics and mechanism of H(2) production and of anaerobic metabolism in general. Expand
A simple energy-conserving system: Proton reduction coupled to proton translocation
It is demonstrated that the production of hydrogen gas by membrane vesicles of P. furiosus is directly coupled to the synthesis of ATP by means of a proton-motive force that has both electrochemical and pH components. Expand
The structure and mechanism of iron-hydrogenases.
  • M. Adams
  • Chemistry, Medicine
  • Biochimica et biophysica acta
  • 5 November 1990
Mechanisms of H2 activation and electron transfer are proposed to explain the effects of CO binding and the ability of one of the hydrogenases to preferentially catalyze H2 oxidation and H2 production. Expand
Tungsten in biological systems.
The first crystal structure of a tungsten- or pterin-containing enzyme, that of aldehyde ferredoxin oxidoreductase from P. furiosus, has revealed a catalytic site with one W atom coordinated to two pterIn molecules which are themselves bridged by a magnesium ion. Expand
Whole-Genome DNA Microarray Analysis of a Hyperthermophile and an Archaeon: Pyrococcus furiosus Grown on Carbohydrates or Peptides
The degree of coordinate regulation revealed by the microarray data was unanticipated and shows that P. furiosus can readily adapt to a change in its primary carbon source. Expand
The unique features of glycolytic pathways in Archaea.
The structure and function of the archaeal glycolytic routes, the participating enzymes and their regulation are re-evaluated by means of integrating results from biochemical and genetic studies with recently obtained comparative and functional genomics data. Expand
Anaerobic microbes: oxygen detoxification without superoxide dismutase.
Unlike superoxide dismutase, the enzyme that protects aerobes from the toxic effects of oxygen, SOR does not catalyze the production of oxygen from superoxide and therefore confers a selective advantage on anaerobes. Expand
Structure of a hyperthermophilic tungstopterin enzyme, aldehyde ferredoxin oxidoreductase
The crystal structure of the tungsten-containing aldehyde ferredoxin oxidoreductase (AOR) from Pyrococcus furiosus, a hyperthermophilic archaeon (formerly archaebacterium) that grows optimally at 100Expand