Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS)
The SAXS pipeline combines automated sample handling of microliter volumes, temperature and anaerobic control, rapid data collection and data analysis, and couples structural analysis with automated archiving to create an efficient pipeline enabling high-throughput analysis of protein structure in solution with small angle X-ray scattering.
The Iron-Hydrogenase of Thermotoga maritima Utilizes Ferredoxin and NADH Synergistically: a New Perspective on Anaerobic Hydrogen Production
The discovery of the bifurcating hydrogenase gives a new perspective on the understanding of the bioenergetics and mechanism of H(2) production and of anaerobic metabolism in general.
High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus.
The structure and mechanism of iron-hydrogenases.
- M. Adams
- ChemistryBiochimica et Biophysica Acta
- 5 November 1990
A simple energy-conserving system: Proton reduction coupled to proton translocation
It is demonstrated that the production of hydrogen gas by membrane vesicles of P. furiosus is directly coupled to the synthesis of ATP by means of a proton-motive force that has both electrochemical and pH components.
Tungsten in biological systems.
The unique features of glycolytic pathways in Archaea.
The structure and function of the archaeal glycolytic routes, the participating enzymes and their regulation are re-evaluated by means of integrating results from biochemical and genetic studies with recently obtained comparative and functional genomics data.
Insights into the Metabolism of Elemental Sulfur by the Hyperthermophilic Archaeon Pyrococcus furiosus: Characterization of a Coenzyme A- Dependent NAD(P)H Sulfur Oxidoreductase
A novel S(0)-reducing system involving NSR and MBX has been found so far only in the heterotrophic Thermococcales and is in contrast to the cytochrome- and quinone-based S( 0)-red reducing system in autotrophic archaea and bacteria.
Whole-Genome DNA Microarray Analysis of a Hyperthermophile and an Archaeon: Pyrococcus furiosus Grown on Carbohydrates or Peptides
The degree of coordinate regulation revealed by the microarray data was unanticipated and shows that P. furiosus can readily adapt to a change in its primary carbon source.
Anaerobic microbes: oxygen detoxification without superoxide dismutase.
Unlike superoxide dismutase, the enzyme that protects aerobes from the toxic effects of oxygen, SOR does not catalyze the production of oxygen from superoxide and therefore confers a selective advantage on anaerobes.