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Purification, crystallisation and characterisation of carboxypeptidase from wheat bran
Abstract A carboxypeptidase was purified and crystallised from wheat bran. Disc gel electrophoresis at pH 4·0 and ultracentrifugal analysis revealed that the enzyme was essentially homogeneous. TheExpand
Stimulation of clover root hair infection by lectin-binding oligosaccharides from the capsular and extracellular polysaccharides of Rhizobium trifolii.
This bacteriophage-induced polysaccharide depolymerase converted the acidic CPS and EPS of R. trifolii 0403 into biologically active oligosaccharides capable of binding trifoliin A and stimulating root hair infection. Expand
Absence of DNA in peroxisomes of Candida tropicalis.
The purification procedure includes the effective conversion of cells to spheroplasts with Zymolyase and sodium sulfite and the separation of the organelles at extremely low ionic strength. Expand
Ligand- and Base-Free Synthesis of 1,3-Diynes Catalyzed by Low Loading of Heterogeneous Pd/C and CuI
A facile and environmentally friendly synthetic method for a variety of symmetrical 1,3-diyne derivatives based on the Pd/C-CuI-catalyzed homocoupling reaction of terminal alkynes has been developed.Expand
Specific phases of root hair attachment in the Rhizobium trifolii-clover symbiosis.
The results are interpreted as a sequence of phases in attachment, beginning with specific reversible interactions between bacterial and plant surfaces (phase I attachment), followed by production of extracellular microfibrils which firmly anchor the bacterium to the root hair (phase 2 adhesion). Expand
Bacteriophage-induced acidic heteropolysaccharide lyases that convert the acidic heteropolysaccharides of Rhizobium trifolii into oligosaccharide units.
The two substrates exhibited different kinetics of depolymerization, and the oligosaccharide products differed in the amount of noncarbohydrate substituents, indicating that the acidic capsular and excreted extracellular polysaccharides from 5-day-old cultures of R. trifolii 0403 were different. Expand
Identification of 3-hydroxybutanoic acid as a component of the acidic extracellular polysaccharide of Rhizobium trifolii 0403
The known non-carbohydrate components of the extracellular polysaccharide of Rhizobium trifolii are acetatelt2, pyruvate1-3, and succinate4. It has been shown that pyruvate substituents are strongExpand
Citbismine-A, -B and -C, New Binary Acridone Alkaloids from Citrus Plants
Three novel dimeric acridone alkaloids named citbismine-A (1), -B (3) and -C (5) have been isolated from the roots of Marsh grapefruit (Citrus paradisi) and Hirado-buntan (C. grandis). The structureExpand
Regeneration and fusion of mycelial protoplasts of Tricholoma matsutake.
Liquid medium for the culture contained glucose, yeast extract and Bacto-Soytone, and the same mediumsupplemented with 0.6m mannitol2 was used as regeneration medium (RM) and both media were adjusted to pH 5.0 before autoclaving. Expand
The chemical structure of acrignine-A (1), an acridone alkaloid carrying lignan moiety, from roots of Citrus plants (Rutaceae) have been elucidated by spectrometric studies using 1H-13C long-rangeExpand