M. V. Stepanova

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C. freundii enteropathogenic strains were found to be capable of producing choleroform thermolabile enterotoxin. Thus, in the study of 96 C. freundii strains 38 enterotoxin-producing cultures (39.5%) were revealed by means of the molecular-biological techniques and 29 such cultures (30.0%), by means of the radioimmunoassay (RIA). 100% coincidence was noted(More)
The content of enterobacteria in fecal samples obtained from 1,146 patients with acute enteric infections was studied. In the first days of the disease about 70% of the patients were found to have abundant and highly diversified intestinal microflora with Escherichia contamination of feces constituting 10(6)-10(7) microbial cells/g in children and(More)
To obtain the information on the genetic control of toxin production in the botulism causative agents, the oligonucleotides were synthesized as the molecular probes by translation of the amino acid sequence of the botulinic type A neurotoxin. The optimal conditions for hybridization of botulinic DNA with the synthetic DNA probes were determined and the(More)
Out of 4,082 Escherichia strains isolated from 989 hospitalized patients with acute enteric infections 2,979 strains belonged to 139 O-groups and the remaining strains could not by typed with O-sera (O1-O164) or were in the serological R-form. 19.3% of the strains isolated from 354 patients showed a positive reaction for thermolabile enterotoxin (TLET)(More)
The conditions of cultivation, ensuring the maximum accumulation of intracellular thermolabile enterotoxin in the cultures of two E. coli strains of different origin, have been studied. Culture media manufactured in the USSR have been selected and the conditions of cultivation, necessary for obtaining intracellular thermolabile enterotoxin in preparative(More)
Accurate mapping of the auroral oval into the equatorial plane is critical for the analysis of aurora and substorm dynamics. Comparison of ion pressure values measured at low altitudes by Defense Meteorological Satellite Program (DMSP) satellites during their crossings of the auroral oval, with plasma pressure values obtained at the equatorial plane from(More)
Different E. coli of 0-groups, enteropathogenic (EPEC), enteroinvasive, enterotoxigenic (ETEC) and diarrheogenic in sporadic child diarrhea (DESCD), regarded as the causative agents of enteric infections, were detected in 70-80% of cases (1,146 E.coli carriers) in the feces of child and adult patients with acute enteric infections (AEI) irrespective of(More)
The modified method for isolation and purification of LT-enterotoxin from Escherichia coli strain H74-114 has been proposed. Modification is based on exclusion of concentration of bacterial cell lysates by 3-5 fold dilution and changing PH during chromatography. The proposed modification permitted to increase the yield of purified and active product 8-10(More)