M. V. Ermolaeva

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The interaction between receptor and a heterotrimeric G protein (alpha beta gamma) is thought to involve the intracellular loops of receptors and specific domains on the G protein. Here we show that a chemically farnesylated peptide (P5far) specific to the carboxyl-terminal domain (amino acids 60-71: DKNPFKELKGGC) of the gamma subunit of the G protein, Gt,(More)
Post-translational prenylation of the carboxyl-terminal cysteine is a characteristic feature of the guanine nucleotide-binding protein (G protein) gamma subunits. Recent findings show that the farnesylated COOH-terminal tail of the gamma 1 subunit is a specific determinant of rhodopsin-transducin coupling. We show here that when synthetic peptides specific(More)
Receptor-G protein interaction is characterized by cycles of association and dissociation. We present evidence which indicates that during receptor-G protein interaction, the C-terminal tail of the G protein gamma subunit, which is masked in the beta gamma complex, is exposed and establishes high-affinity contact with the receptor. This potential(More)
AIM Assessment of the dynamic surface tension (DST) of blood serum (BS), synovial fluid (SF) in various courses of rheumatoid arthritis (RA). MATERIALS AND METHODS Forty three patients with RA and 63 apparently healthy individuals were examined. DST of BS and SF was determined in the computer-aided tensiometer and some blood biochemical parameters were(More)
Dependencies of larch (Larix sibirica Ledeb.) growth and reproductive organ formation on its infestation by a phyllophagous miner, larch casebearer (Coleophora sibiricella Flkv., Lepidoptera, Coleophoridae) were studied. The work was performed in the sustained outbreak of the larch casebearer in the forest-steppe-larch forest at Kuznetsk Alatau. For the(More)
The condition was studied of the dynamic surface tension of biologic fluids in patients with rheumatoid arthritis using a method of maximum pressure in the vesicle with the aid of computerized tensiometer MPT-1 "Lauda" (Germany). Rise in indices of blood serum surface tension with diminution of the slope of tensiogram curves may suggest the development of(More)
A method for purifying DNA-specific catalytic antibodies based on affinity chromatography on protein A Sepharose and on both modified and non-modified DNA-cellulose as well as HPLC has been developed. The elution conditions with high yields of DNA-hydrolyzing activity of antibodies have been optimized. The biochemical and immunological properties of(More)
Dynamic surface-tension (S-T) was investigated of blood and urine of healthy individuals and patients with chronic glomerulonephritis by the proposed method of maximum pressure in a bubble with the aid of the computer-assisted device tensometer MPT-1 (Lauda, Germany). Patterns of changes in dynamic and static S-T were found out as were those of the slope of(More)
A protein-inhibiting LAK cell-mediated cytotoxicity was isolated from a LAK cell-conditioned medium. The N-terminal amino acid sequence of this protein appeared to be identical to fetal calf fetuin. Pure isolated fetuin, as well as commercially available preparations of this protein, were shown to inhibit cytotoxic activity of both cytotoxic proteins(More)
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