M. Sandbacka

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There is a need to replace acute toxicity tests on fish (LC(50)) with more cost effective assays. The main objective of this study was to explore whether gill epithelial cells, hepatocytes and Daphnia magna could be used to predict acute toxicity of surfactants on fish. The acute toxicity of 10 synthetic surfactants (anionic, cationic, nonionic and(More)
Communication across gap junctions between cells in various tissues is considered an important mechanism for control of cellular growth, differentiation and function. Although cell-cell coupling in the gill epithelium is likely for functional reasons, the common view is that gap junctions are not present between cells in the gill epithelium of teleostean(More)
The large number of chemicals in our environment, many of which have been insufficiently tested for toxicity, justifies the need for rapid and reliable in vitro testing to predict toxicity. In aquatic toxicology the use of cellular toxicity tests has been limited. In the present study, the usefulness of freshly isolated gill epithelial cells from rainbow(More)
The objective of this study was to characterize rainbow trout (Oncorhynchus mykiss) gill epithelial cells in primary culture by evaluating their ability to maintain glutathione and glucuronide conjugating enzymes. The activity and inducibility of the phase II enzymes was investigated as a function of culture time. Glutathione S-transferase (GST) and(More)
Owing to an error in the production process the afilliation of the above-mentioned article has been mistakenly interchanged. The affilation as it should have been printed is presented below: Department of Biology and ∗Department of Biochemistry, Åbo Akademi University, BioCity, Artilleriegatan 6, 20520 Turku/Åbo, Finland; (Phone: +358 21 654 065. Fax: +358(More)
Gill epithelial cells are less sensitive than fish for most test chemicals, but a high correlation and a slope of the regression line close to 1 support the use of gill epithelial cells for prediction of acute toxicity in fish. Cells in suspension perform as well as cultured cells in the toxicity tests. However, the use of cells in suspension results in a(More)
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