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We examined properties of chemical neurotransmission at the level of primary afferent inputs into spinal cord with the new easy-to-use in vitro model of contiguously-cultivated dissociated both the dorsal root ganglion neurons (DRG) and the dorsal horn spinal cord neurons (DHSC) from newborn rats. The results of our studies showed the presence of excitatory(More)
Potassium channels make up the largest family of voltage-activated channels and play a key role in maintenance of cell excitability and in transmission of information within the nervous system. The aim of this work was to find out the specific subtypes of voltage-activated potassium channels peculiar to GABAergic interneurons of rat hippocampal culture. It(More)
Electrophysiological characteristics of hippocampal neurones cultured with Pro-Gly-Pro peptide were studied using glutamate excitotoxisity model (excitotoxic damage was induced by 100 mkM glutamate application during 5 min). It was found that negative changes in neurones cultured with 10 mcM Pro-Gly-Pro were less prominent if compared with control ones.(More)
The rat superior cervical ganglion (SCG) neurones demonstrate responses on the threshold stimulation of the cervical sympathetic nerve, extrinsic (ECN) and intrinsic (ICN) carotid nerves. Both antidromic and synaptic responses were evoked by antidromic stimulation of ECN and ICN. The synaptic responses of each neuron differ by great variability amplitudes(More)
On rat retinal preparation in whole-cell mode retinal ganglion cells (RGC) tonic firing induced calcium signals were recorded and their modulation by Kv3 potassium channels was studied. RGC depolarization with 500 ms current steps caused tonic action potentials (AP) generation and development of calcium signals which amplitude depended quite linearly on(More)
In the present study, we investigated the sensitivity of GABAergic short-term plasticity to the selective P- and P/Q-type calcium channels blocker omega-agatoxin-IVA. To block the P-type channels we used 30 nM of this toxin and 200 nM of the toxin was used to block the P/Q channel types. The evoked inhibitory postsynaptic currents (eIPSC) were studied using(More)
On isolated retinal preparation from STZ-induced diabetic rats in whole-cell configuration the basic electrical properties of retinal ganglion cells (RGC) were studied. It was shown that on 8th week after STZ maximal depolarization-induced firing frequency is reduced and decay time constant of high-frequency stimulation-induced calcium signals is increased(More)
Electrophysiological properties of inhibitory (GABAergic) neurones were studied in dissociated hippocampal culture using simultaneous whole cell recordings from pairs of monosynaptically coupled neurons. Reliable identification of GABAergic neuron was performed by presence of monosynaptic inhibitory currents at postsynaptic cell in response to action(More)
Dynamic changes in the intracellular free Ca2+ concentration ([Ca2+]i) were studied in hippocampal cultured neurons using fluorescent Ca(2+)-indicator dye Indo-1 and somatic whole-cell recordings. During the tetanus stimulation Ca(2+)-transient increased their amplitude up to a steady-state level during repetitive stimulation. We identified two groups of(More)
High frequency firing properties of the rat retinal ganglion cells and role of 4-AP-sensitive potassium current in firing generation were studied using whole cell patch-clamp techniques. Potassium channel blocker 4-AP (0,2 mM) reduced depolarization-evoked firing rate by 62% (from 50 +/- 5, n = 12 to 19 +/- 2 Hz, n = 7). Single action potential parameters(More)