M. S. Boyanova

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UNLABELLED Our purpose was to investigate the diagnostic value of the RNA-based HPV test (NucliSENS EasyQtm HPV) which utilizes NASBA technology for determination of the expression of E6/E7 of five high-risk HPV types (16, 18, 31, 33 u 45). METHODS Two hundred women aged 16 to 61 (mean 34.44) were prospectively studied. All of the women were subject to(More)
Until recently, nonenzymatic glycosylation (glycation) was thought to affect the proteins of long living eukaryotes only. However, in a recent study (Mironova, R., Niwa, T., Hayashi, H., Dimitrova, R., and Ivanov, I. (2001) Mol. Microbiol. 39, 1061-1068), we have shown that glycation takes place in Escherichia coli as well. In the present study, we(More)
For the study of functional domains of hIFNgamma, two MABs were characterized. Both MABs, named 1E11 and 5D6, were sequence-specific for hIFNgamma. According to the estimated additivity index, they recognized epitopes located at distinct non-overlapping areas of the hIFNgamma molecule. When pre-incubated with hIFNgamma, MAB 1E11 was able to neutralize the(More)
Glycation of recombinant human interferon-gamma (rhIFN-γ) causes conformational alterations of the molecule and results in reduction of its biologic activity. The aim of this study was to find adequate approaches for prevention of glycation in order to obtain a stable recombinant protein with sustained biologic activity. To this end we investigated the(More)
The significance of the C-terminal part of human interferon gamma (hIFNgamma) for its biological activity was studied by 3(')-end gene mutagenesis. A series of nine derivative genes obtained by systemic deletion of three codons was constructed and expressed in Escherichia coli LE392. It was shown that the yield of recombinant protein gradually decreased and(More)
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