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  • M Reza Ghassemifar, Bhavwanti Sheth, Tom Papenbrock, Henry J. Leese, Franchesca D. Houghton, Tom P. Fleming
  • Biology, Medicine
  • Journal of cell science
  • 2002 (First Published: 1 August 2002)
  • The tight junction protein occludin possesses four transmembrane domains, two extracellular loops, and cytoplasmic N- and C-termini. Reverse transcription-PCR analysis of human tissues, embryos andContinue Reading
  • Roy W. Tarnuzzer, S P Macauley, +9 authors Gregory Scott Schultz
  • Biology, Medicine
  • BioTechniques
  • 1996 (First Published: 1 April 1996)
  • Detection of low-abundance mRNAs by reverse transcription-polymerase chain reaction (RT-PCR) has become a standard technique to determine gene expression by tissues and cells in culture. The abilityContinue Reading
  • M Reza Ghassemifar, Judith J. Eckert, Franchesca D. Houghton, Helen M. Picton, Henry J. Leese, Tom P. Fleming
  • Biology, Medicine
  • Molecular human reproduction
  • 2003 (First Published: 1 May 2003)
  • We investigated gene expression associated with trophectoderm epithelial intercellular junction formation in single human embryos at different stages of cleavage using RT-PCR methods based uponContinue Reading