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We have previously constructed a cDNA clone pHCALl, covering most of the C-terminal propeptide domain of human proal(I) collagen mSNA, by inserting a 678 bp EcoRI-XhoI fragment of cDNA into pBR322 (1). Since the Xhol/Sall ligation prevented removal of the insert, we used the same strategy to obtain a similar clone in pUC8. RNA was isolated from fetal(More)
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