M. R. Chailakhyan

  • Citations Per Year
Learn More
In the present study we carried out experiments in vitro and in vivo and investigated the effect of proline-rich polypeptide (PRP) on the proliferation and effectiveness of colony formation of MMSCs in vitro. Various routes and doses of PRP administration to rats increased the number of MMSCs in bone marrow and spleen. Our research revealed opposite effects(More)
We present the results of a study on the proliferative and differentiation potential of individual clones of stromal fibroblasts growing in monolayer cultures of bone marrow cells. Each precursor cell yielding a large colony in primary culture is capable of up to 34 doublings in vitro. The transplantation of clones or monoclonal strains of stromal(More)
This paper describes our study on the regeneration of hemopoietic and stromal components of bone marrow after mechanically emptying the medullar cavity of the guinea pig tibia. The intensity of hemopoiesis was determined from the number of hemopoietic cells, while the concentration and total number of stromal precursor cells were used to estimate the(More)
The aim of the study was to assess the effect of proline-rich polypeptide (PRP) on bone marrow stromal stem cells in vivo and in vitro and on tumour cell lines. Methods. Isolation of giant-cell tumour (GCT) stromal cells and obtaining these cell strains; obtaining normal bone marrow stromal cell strains; PRP administration to rats; bone marrow cell(More)
The model of cultivation of mouse, guinea-pig and rabbit stromal cell-precursors has been developed [2, 4, 6]. It has been shown that the main factors affecting the fibroblast cloning efficiency (CEf) are the initial explantation density of cell suspensions (providing the conditions for the adhesion of CFUf) and growth factors (PDGF, EGF, TGF, IGF-1, etc.),(More)
  • 1