Learn More
  • G. Landemore, M. Quillec, S. -E. Letaïef, J. Izard
  • 1994
This study deals with the ultrastructure of the chondroitin sulphate proteoglycans of the Kurloff body, a large lysosome organelle, metachromatic towards Toluidine Blue, of a blood cell unique to the guinea pig and called the Kurloff cell. Splenic Kurloff cell from oestrogen-treated guinea pig cells were examined after staining with Cuprolinic Blue, a(More)
In order to study their natural killer effect, guinea pig splenic Kurloff cells were fractionated by Percoll discontinuous density gradient centrifugation. Kurloff cells were collected and tested for cytotoxicity in a 24-hr chromium-release test. Comparison of different splenic cellular samples (of males or estrogenized females) with increasing percentage(More)
  • G. Landemore, M. Quillec, N. Oulhaj, J. Izard
  • 1991
In the course of an ultrastructural cytochemical study of intracellular sulphated proteoglycans involving the addition of cetylpyridinium chloride in the primary aldehyde fixative, a remarkable ultrastructural preservation of the collagenassociated sulphated proteoglycans was observed. Together with the preservation of their localization among the collagen(More)
Large populations of splenic Kurloff (150 - 200 X 10(6) Kurloff cells) were obtained from estrogenized guinea pigs by isopycnic centrifugation in a Percoll solution of 1.085 g/ml starting density. The Kurloff cells settled at a buoyant density of about 1.100 g/ml. The purity of these cell suspensions reached 95%, as assessed by phase contrast microscopy and(More)
Kurloff cells belong to the group of macrophages as far as ultrastructure, adhesiveness and identification with Kupffer cells (in the case of the liver) are concerned. A characteristic group of features makes it easy to recognize them: PAS reaction, cyanol-blue staining, presence of myelin figures in electron microscopy and attachment to glass-slides.(More)
  • G. Landemore, M. Quillec, N. Oulhaj, J. Izard
  • 1993
This study deals with the ultrastructure of the chondroitin sulphate proteoglycans of the Kurloff body, a large lysosomal organelle that stains metachromatically with Toluidine Blue and which is present in Kurloff cells (a blood cell unique to the guinea pig). Splenic tissues were fixed with 1% cetylpyridinium chloride (CPC) added to 4% paraformaldehyde and(More)
L2C leukemia is a leukemia that occurs in strain two guinea pigs. The L2C cells are natural killer-sensitive. The Kurloff cell (KC), a guinea pig NK cell, develops a 3-fold increase in lysosomal enzyme activity and the number of KC cells increases during leukemogenesis, leading to KC cell-mediated L2C cytolysis. This paper shows that conjugates are produced(More)
We made red cell and white cell counts in an homogeneous sample of more than 250 healthy, non gravid, adult, female guinea-pigs. Up to the present it is the highest number of guinea-pigs on which systematic blood counts have been taken. Each category of cells was studied statistically. Our mean of the red cells (5.03 X 106/mm3) is close to most of the means(More)
  • Prof. Dr. J. Izard, M. T. Barrellier, M. Quillec
  • 2004
Kurloff cells belong to the group of macrophages as far as ultrastructure, adhesiveness and identification with Kupffer cells (in the case of the liver) are concerned. A characteristic group of features makes it easy to recognize them: PAS reaction, cyanol-blue staining, presence of myelin figures in electron microscopy and attachment to glass-slides.(More)
We have investigated the occurrence of fibrinogen or its derivatives in the Kurloff bodies of female guinea pigs by means of immunofluorescence, histochemistry and ultrastructural analysis. The Kurloff bodies are fluorescent after incubation with anti-guinea pig fibrinogen immuno-globulins coupled with fluorescein isothiocyanate. PTAH, Martius scarlet blue,(More)