M. Quillec

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To document the ultrastructural distribution of lens capsule proteoglycans, rabbit lens capsules were fixed and stained overnight in 50 mM sodium acetate, pH 5.6, containing 2.5% glutaraldehyde, 0.2% Cuprolinic Blue and 0.2 M MgCl2. They were rinsed, stained with 1% aqueous sodium tungstate, embedded in Epon, sectioned (60 nm), and examined with an electron(More)
Very high pressure freezing and cryosubstitution of Kurloff cells preserves the ultrastructural morphology of Kurloff bodies, particularly the myelin figures, as shown by embedding in epoxy resin and conventional postembedding staining. It also preserves the Kurloff body proteoglycans as more expanded spindle-like shapes than does fixation with formaldehyde(More)
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