M. Michulec

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The growth and ochratoxin A producing activity at the phenotypical and molecular level ofP. verrucosum in wheat stored at different relative moisture conditions have been followed. Growth has been measured by determination of colony forming units (cfu). The expression of the ochratoxin A polyketide synthase (otapksPV) has been followed over time by reverse(More)
Based on the sequence of the ochratoxin A polyketide synthase gene (otapksPV), a polymerase chain reaction (PCR) system for the specific detection of Penicillium verrucosum in wheat has been developed. In a further approach, a real-time PCR system has been applied to determine the growth kinetics of P. verrucosum in wheat at cell numbers above 10(3)(More)
The application of headspace solid-phase microextraction for isolation and enrichment of solvent residues from oils and pharmaceuticals is discussed. The optimal parameters for isolation and preconcentration of common process solvents (hexane, benzene, toluene and selected chloroderivatives of hydrocarbons) were established. Four fiber types (100 microm(More)
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