M. Margarida. Oliveira

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Eight new cDNA sequences for S-RNases were cloned and analysed from almond (Prunus dulcis) cultivars of European origin, and compared to published sequences from other Rosaceae species. Insertions/deletions of 10–20 amino acid residues were detected in the RC4 and C5 domains of S-RNases from almond and sweet cherry. The S-RNases of the Prunus species and(More)
Almond (Prunus dulcis) displays gametophytic self-incompatibility. In the work reported here, we cloned two novel S-RNase genes from almond cultivar Ferragnès (genotype S1S3) using PCR. The S1-RNase gene has the same coding region as the Sb gene cloned from almond cultivated in the USA; however, their introns are different in sequence. S1 was cloned and(More)
A cDNA for an S-like RNase (RNase PD2) has been isolated from a pistil cDNA library of Prunus dulcis cv. Ferragnés. The cDNA encodes an acidic protein of 226 amino acid residues with a molecular weight of 25 kDa. A potential N-glycosylation site is present at the N-terminus in RNase PD2. A signal peptide of 23 amino acid residues and a transmembrane domain(More)
In order to determine the suitability of reference or housekeeping genes as internal controls in real-time reverse transcriptase PCR (RT-PCR) assays for quantification of target mRNAs, we studied the levels of expression of four candidate reference genes in maritime pine by real-time RT-PCR. The expression levels obtained for(More)
Rab-related small GTP-binding proteins are known to be involved in the regulation of the vesicular transport system in eukaryotic cells. We report the characterization of a previously isolated full-length cDNA PpRab1 from Pinus pinaster. Amino acid sequence analysis revealed the presence of G1–G5 conserved domains of the GTPase Ras superfamily and a double(More)
Differential display of Poly (A+) RNA isolated from zygotic embryos of maritime pine (Pinus pinaster) in 9 stages of development was used to isolate several cDNAs of differentially expressed genes during embryo development. Many cDNA sequences share low similarities to any known DNA or protein sequences but 5 transcripts are predicted to encode homologues(More)
Background and objectives Cork oak (Quercus suber L.) is one of the dominant broadleaved woody species in the western Mediterranean Basin, defining unique open woods. These woodlands have an outstanding economical and ecological value in this region, particularly in Portugal, where they sustain a strong cork industry. In the context of a prospective(More)
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