M. Madhusudan

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B. N-Terminus/C-Terminus 2253 1. N-Terminal Tail 2253 2. C-Terminal Tail 2257 C. Signal Integration Motifs 2257 1. C Helix 2258 2. Activation Loop 2258 3. F Helix 2258 D. Dynamics 2259 1. Fluorescence Anisotropy 2259 2. Molecular Dynamic Simulations 2260 III. Inhibitors of the Catalytic Subunit 2260 A. Protein Kinase Inhibitor 2260 B. Regulatory Subunits(More)
Spo0F is a secondary messenger in the "two-component" system controlling the sporulation of Bacillus subtilis. Spo0F, like the chemotaxis protein CheY, is a single-domain protein homologous to the N-terminal activator domain of the response regulators. We recently reported the crystal structure of a phosphatase-resistant mutant Y13S of Spo0F with Ca2+ bound(More)
For the last three decades, a large scale integration of distributed generation (DG) is beginning to change the electrical distribution network from passive to active. Consequently, technical difficulties are created by significant impacts generated by DGs with voltage variation being the dominant effect. This paper deals with operation and control of a(More)
Two-component signaling systems are used by bacteria, plants, and lower eukaryotes to adapt to environmental changes. The first component, a protein kinase, responds to a signal by phosphorylating the second component; a response regulator protein that often acts by inducing the expression of specific genes. Response regulators also have an autophosphatase(More)
An aqueous two phase system of polyethylene glycol (PEG) and salts was evaluated for separation and purification of alkaline proteases from chicken intestine. Among the different salts evaluated potassium phosphate and sodium citrate gave higher enzyme yield (73.5% and 69.7% respectively) and enzyme purification (5.3 and 7.4 fold) in PEG rich upper phase.(More)
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