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The adipose tissue-derived hormone leptin regulates body weight homeostasis by decreasing food intake and increasing energy expenditure. The weight-reducing action of leptin is thought to be mediated primarily by signal transduction through the leptin receptor (LR) in the hypothalamus. We have used immunohistochemistry to localize LR-immunoreactive (LR-IR)(More)
Leptin is an adipose tissue-derived hormone that regulates body weight via interactions with hypothalamic neuronal circuitries expressing specific leptin receptors (Ob-R). The Ob-Rs act via the JAK-STAT (Janus kinase-signal transducers and activators of transcription) pathway of signal transduction. Recent evidence suggests that primarily the transcription(More)
The obese phentotype of ob/ob mice is linked to a mutation in the ob gene that results in expression of a truncated inactive protein. The ob gene product, leptin, is synthesized in adipose tissue and is a circulating factor that regulates body weight. Leptin receptors were recently cloned and a mutation in the leptin receptor gene in obese db/db mice was(More)
Hypocretins/orexins are recently characterized peptides that are synthesized in neurones of the lateral hypohalamus and stimulate food intake in rats. To clarify whether leptin may interact with hypocretin/orexin to reduce ingestive behaviour, the presence of leptin receptor-immunoreactivity in hypocretin/orexin-containing neurones was examined. Many leptin(More)
The effect of leptin on gastric emptying of glucose was studied in freely moving rats bearing intragastric fistulas. Leptin (0.39 microg and 3.9 microg) injected into the fourth ventricle inhibited gastric emptying significantly, whereas s.c. administration of leptin (10 microg/kg) had no effect. Leptin receptor immunoreactivity, revealed by an antiserum(More)
A family of novel hypothalamus-specific peptides called orexins have recently been discovered and characterized. The orexins stimulate appetite (the greek word orexis means appetite) when given intraventricularly to rats. Their genes are expressed bilaterally in the lateral hypothalamus, a region previously known to regulate food intake. The two peptides,(More)
Botulinum neurotoxin F (BoNTx F) is a zinc-dependent endopeptidase that causes proteolytic cleavage of the vesicle protein VAMP (vesicle-associated membrane protein). VAMP is an important component of the molecular machinery regulating docking and fusion of secretory vesicles with the target membrane. We have investigated presence of VAMP protein in(More)
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