M J Parry
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1. An 870-fold purification of glucokinase from rat liver is described which involves ammonium sulphate fractionation and the use of DEAE-Sephadex, DEAE-cellulose and polyacrylamide columns. 2. The… Continue Reading
1. Magnesium ions are the most effective bivalent ions in the glucokinase reaction. 2. The molecular weight of rat hepatic glucokinase is 48000-49000 as assessed by gel filtration on Sephadex G-100.… Continue Reading
Publisher Summary This chapter discusses the determination of glucokinase from liver. In the course of assay, a known volume of the enzyme preparation is added to a cuvette, having a light path of 1… Continue Reading