M. Hupková

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A strain of Pseudomonas aeruginosa No. 4870 was isolated at the Pediatric University Hospital in Bratislava which transferred, by conjugation, the resistance to cefotaxime (CTX), ceftazidime (CTZ) and aztreonam (AZA) and to more classical anti-pseudomonadal antibiotics. This strain was also lysogenic and contained a temperate bacteriophage which was(More)
Two wild-type bacteriophages, designated AP-2 (for P. aeruginosa phage) and AP-12, have been isolated and propagated from two multiple drug resistant strains of P. aeruginosa. Both phages were found to transduce Imipenem- (IMI), Aztreonam- (AZA) and Ceftazidime- (CTZ) resistance as well as resistance determinants to other drugs. Genetic analysis showed that(More)
The lysate of phage F-116, propagated in a multiple drug resistant clinical isolate of Pseudomonas aeruginosa No. 131 was used to transduce determinants of antibiotic resistance to susceptible auxotrophic laboratory strains of the same species. The phage preparation, designated F-116/131 was found to transduce four determinants of resistance, i.e. to(More)
We monitored systematically, for more than five years, the eventual transferability of resistance to imipenem in strains of Pseudomonas aeruginosa isolated from patients in Frankfurt University Clinics. Quite recently, four strains have been found which transfer resistance to imipenem to recipient strains of P. aeruginosa. Although in three strains imipenem(More)
Flavobacterium odoratum (1) has been found to hydrolyse imipenem due to production of a metallo-13-1actamase (2, 3). In September 1993 we obtained two isolates of Flavobacterium rneningosepticurn successively from the sputum of a seriously ill patient in an intensive care unit of the clinic of neurology of a large university hospital in Bratislava (Slovak(More)
Transferable resistance to cefotaxime was demonstrated in 21 nosocomial strains of Klebsiella pneumoniae and Escherichia coli subsequently isolated from patients in two large University clinics. Using the double-disk diffusion test, we could detect, in each such strain, as well as in E. coli 3110 K-12 transconjugants after the transfer, the production of an(More)
This paper examines the development of sacred structures in rural Czechia (former Czechoslovakia). Sacred structures could be associated with the creation of important symbols and distinctive rural regional identities in Czechia. Rural Czechia is not uniform: it represents several different rural landscapes. This paper considers whether sacred structures(More)
Generalized transducting Pseudomonas aeruginosa phage F-116 was propagated in an Imipenem (IMI)-resistant clinical isolate of P. aeruginosa No. 191 and the phage was then used to transduce the IMI-resistance to a susceptible auxotrophic mutant strain of the same species. Transduction seems to be a suitable method for study of resistance determinant(s) and(More)
Due to world-wide use of 3rd generation cephalosporins and new beta-lactam antibiotics (imipenem, aztreonam), serious problems emerge caused by the appearance and spread of bacterial strains resistant also to these drugs. While the resistance to ceftazidime, cefotaxime and other "stable" cephalosporins has a transferable character and is plasmid-coded, the(More)