M. Germin Nisha

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A gene encoding amylopullulanase (gt-apu) of the extremely thermophilic Geobacillus thermoleovorans NP33 was cloned and expressed in Escherichia coli. The gene has an open reading frame of 4,965 bp that encodes a protein of 1,655 amino acids with molecular mass of 182 kDa. The six conserved regions, characteristic of GH13 family, have been detected in(More)
In order to understand the role of N1 domain (1–257 aa) in the amylopullulanase (gt-apu) of the extremely thermophilic bacterium Geobacillus thermoleovorans NP33, N1 deletion construct (gt-apuΔN) has been generated and expressed in Escherichia coli. The truncated amylopullulanase (gt-apuΔN) exhibits similar pH and temperature optima like gt-apu, but(More)
The pink stem borer Sesamia inferens (Walker) (Lepidoptera: Noctuidae) has attained pest status in recent years in the world collection of sugarcane germplasm maintained at the Sugarcane Breeding Institute Research Centre, Kannur, Kerala state, India. With a view to identify sources of resistance to the hitherto minor pest, which occurs during the early(More)
  • Mrs Nisha, V Vader, V P M S Polytechnic, Thane, Sr V P M Lecturer, Thane S Polytechnic
  • 2011
Electrical energy is universally accepted as an essential commodity for human beings. Energy is the prime mover of economic growth and is vital to the sustenance of a modern economy. Future economic growth crucially depends on the long-term availability of energy from sources. Areas of application of Energy Conservation are Power Generating Station,(More)
Pullulan hydrolyzing enzymes are endoacting, classified based on the substrate specificity and hydrolysis products as pullulanases (type I and II) and pullulan hydrolases (type I, II and III). Pullulanases and pullulan hydrolase type I are produced by bacteria and archaea. Among bacteria, many mesophilic, thermophilic and hyperthermophilic bacteria produce(More)
The 3′-deleted amylopullulanase gene from the extreme thermophile Geobacillus thermoleovorans (Gt-apuΔC) was expressed extracellularly in Pichia pastoris under both methanol-inducible AOX1 and constitutive GAP promoters. The expression of the gene (Gt-apuΔC) was higher under GAP promoter (36.2 U ml−1, α-amylase; 33.5 U ml−1, pullulanase) than that under(More)
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