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The secondary structure of dendrotoxin I, an important constituent of the venom of the African black mamba snake Dendroaspis polylepis polylepis, was determined in aqueous solution by two-dimensional methods. Complete sequence-specific 1H-NMR assignment was obtained with the exception of the backbone amide proton of Gly39 and Cys40. Dendrotoxin I is based… (More)
The efficiency of selective refocusing pulses to suppress J modulation of spin echoes when using the surface-coil technique has been investigated. 31P T2 values obtained for ATP by the HAHN spin-echo method remain in good agreement when the standard refocusing pulse is replaced by selective pulses based on the DANTE excitation method.
31P NMR spin-lattice (T1) and spin-spin (T2) relaxation times of phosphocreatine, ATP, inorganic phosphate, and phosphomonoesters have been measured in vivo at 4.7 T in rat brain and rat brain tumors implanted on nude mice. The relaxation data were acquired using a phase-cycled saturation-recovery spin-echo sequence. The problems associated with the phase… (More)