M. F. Dixon

P. Quirke7
J. E. Dyson2
F. A. Lewis2
S. M. Bell2
C. C. Bird2
7P. Quirke
2J. E. Dyson
2F. A. Lewis
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In order to investigate allele loss in colorectal tumours we have developed a rapid technique which overcomes most of the problems associated with radioactive Restriction Fragment Length Polymorphism (RFLP) analysis of allele loss. We utilise microsatellite length polymorphisms which are highly informative and are closely linked to loci of interest.(More)
p53 protein was detected by immunohistochemistry in 42% of 52 colorectal adenocarcinomas. Positive tumours were significantly more frequent in the distal colon, and demonstrated a higher rate of cell proliferation. No correlation was found with tumour grade, Dukes' stage, presence of DNA aneuploidy or patient survival. The role of p53 in colorectal(More)
Flow cytometry and histopathology were utilised in evaluating 50 primary and 16 metastatic colorectal carcinomas to determine the influence of heterogeneity and proportion of dying cells on pathological assessments. A new procedure was developed for staining unfixed whole cells with acridine orange and ethidium bromide to quantify DNA and RNA content and(More)
One hundred and nine samples comprising carcinomas, adenomas, dysplastic, inflamed and normal mucosa from patients with sporadic colon cancer and ulcerative colitis (UC) were analysed for c-Ki-ras mutations. DNA was extracted from archival paraffin-embedded material, amplified using the polymerase chain reaction (PCR) and the PCR products analysed using(More)
Several techniques exist for the assessment of cell proliferation in cell suspensions and tissues. These include counting of mitotic figures; labelling with tritiated thymidine or bromodeoxyuridine, and DNA flow cytometry. The latter is a particularly versatile technique applicable to both fresh and formalin fixed paraffin embedded tissue, and its use in(More)
There is increasing evidence that abnormalities of cellular DNA within primary tumours are of prog-nostic significance. Deviation from the normal diploid DNA content of tumour cells has been associated with a poorer prognosis in carcinomas of the breast (Friedlander et al., 1984a), ovary (Friedlander et al., 1984b) and colon (Wolley et al., 1982). However,(More)
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