M. F. A. Goosen

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Controlled antibiotic release films, melt-extruded cylinders, and suspension-extruded/coated cylinders were manufactured from biodegradable poly(D,L-lactide) (PDLLA) and poly(D,L-lactide-co-epsilon-caprolactone). These devices have potential application in the treatment of osteomyelitis. The in-vitro release properties of the devices were examined with drug(More)
To gain better insight into mass transfer problems in encapsulated cell systems requires a combination of experimental investigations and mathematical modeling. Specific mass transfer studies are reviewed including oxygen transfer in immobilized animal cell culture bioreactors, modeling of polymer droplet formation and encapsulated animal cell growth, and(More)
The development of insect cell suspension culture techniques for the production of insect pathogenic viruses and recombinant proteins has been reviewed, with an emphasis on process scale-up and reactor design considerations. The problems of culture media cost and insect cell shear sensitivity have also been addressed.
A two-dimensional mathematical model for animal cell growth was employed to study the suspension, as well as stationary, culture of micro-encapsulated and gel immobilized animal cells. For stationary microcapsules with low-viscosity intracapsular liquid, it was found that capsule radius, capsule loading and medium-change time have the most significant(More)
Insect cells (Spodoptera Frugiperda), infected with a temperature-sensitive mutant (TS10) of theAutographa Californica nuclear polyhedrosis virus (AcNPV), were cultured in multiple membrane alginate-polylysine (PLL) microcapsules. It was possible to obtain intracapsular cell densities of 8× 107 cells/mL of capsules and virus concentrations of up to 109(More)
Polymer microbead production by parallel plate electrostatic extrusion is presented. Factors affecting microbead size such as needle gauge, electrostatic potential, distance between needle and collecting solution, and polymer solution concentration and flow rate were evaluated. Smaller microbeads resulted from reduced needle diameter, reduced needle to(More)
The performance of an external loop air-lift bioreactor was investigated by assessing the inter-relationships between various hydrodynamic properties and mass transfer. The feasibility of using this bioreactor for the production of monoclonal antibodies by mouse hybridoma cells immobilized in calcium alginate gel beads and alginate/poly-l-lysine(More)
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