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We describe a method for perfusion of a working isolated rat heart with washed erythrocytes suspended in a Krebs-Henseleit bicarbonate buffer containing bovine albumin (fraction V). With washed pig red cells, as hematocrit was varied between 0 and 40%, coronary flow (CF), aortic flow (AF), external work (W), and myocardial oxygen consumption (MVO2) were(More)
This study examines the transient response of coronary blood flow to acute changes in O2 content at normal and high arterial PO2 (Pa, O2) in the blood-perfused, working isolated rat heart. The perfusion system used in this study presents the following advantages: it eliminates the gas/blood interface, includes a peripheral circulation for control of(More)
Hemodynamic and metabolic adaptations of isolated working heart perfused alternatively with normal or low oxygen carrying capacity medium were studied in an experimental model. A step change in arterial oxygen content (1.75 to 15.3 ml O2/100 ml) was followed by a decrease in coronary flow, an increase in aortic flow, external work, myocardial oxygen(More)
A theoretical study was developed to evaluate effects of changes in hematocrit on red cell flow partitioning at narrow capillary bifurcations of unequal diameters. Non-Newtonian blood viscosity and non-linear relationships between blood and red cell flows at capillary bifurcations are taken into account. Results suggest that heterogeneity of diameters is a(More)
The effect of an increase in hemoglobin O2 affinity on myocardial O2 delivery was studied in a blood perfused working rat heart preparation. In a first series of experiments P50 (PO2 for which saturation is 50%) was lowered by use of carbon monoxide. The heart was alternatively perfused with the blood sample of P50 = 32 mm Hg and the blood sample of P50 =(More)
The risk of contracting certain disorders following a blood transfusion is currently becoming worrisome not only for physicians who are aware of this problem, but also for patients who, with increased media attention on AIDS, are more afraid of this potential risk than of the surgical or anesthetic risks. In fact, hepatitis constitute the major risk(More)