M. C. Chamblier

Learn More
Insulin receptors have been demonstrated in isolated rat intestinal epithelial cells. The specific binding of 125I-insulin was time — and temperature — dependent, the optimal temperature of study being 15°. Dissociation of bound 125I-insulin by an excess of unlabelled hormone was rapid and attained 66±2% in 2 h. When initiated by dilution, the dissociation(More)
Physiological studies indicate that epidermal growth factor-urogastrone (EGF) acts on stomach epithelium as mitogen and modulator of acid secretion. Here, we studied the binding of 125I-EGF to gastric glands isolated from the guinea-pig fundus (acid-secreting part) and antrum. At 20 degrees C, the association of 125I-EGF to gastric glands was time-dependent(More)
The biological action and binding of insulin were tested in two human intestinal cancer cell lines originating from the duodenum (HUTU 80) and the colon (HT 29). After serum deprivation for 24 hr, insulin stimulated cell division and the incorporation of labeled precursors into RNA, protein, and DMA for both cell lines. The action on the RNA and protein was(More)
We examined the effect of diabetes on protein synthesis in intestinal epithelial cells. Isolated enterocytes of diabetic rats display a marked decrease (50%) in 3H-leucine incorporation into intestinal proteins as compared to normal cells. Treatment of diabetics with insulin restores the level of protein synthesis up to normal values. Similarly, in vitro(More)
An homogeneous cell population isolated from the inguinal tissue of 3-day-old rats is able to proliferate in primary culture. In the presence of a physiological concentration of insulin (1.5 nM) it converts into cells exhibiting the morphology and the biochemical characteristics of adipocytes. Insulin and epidermal growth factor (EGF) receptors were studied(More)
The biological action and binding of insulin were tested in two human intestinal cancer cell lines originating from the duodenum (HUTU 80) and the colon (HT 29). After serum deprivation for 24 hr, insulin stimulated cell division and the incorporation of labeled precursors into RNA, protein, and DNA for both cell lines. The action on the RNA and protein was(More)
  • 1