M B Kupletskaia

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A new procedure for isolation of tyrosine-phenol-lyase from the cells of C. freundii strain 62 allowing to obtain a highly purified enzyme with a high yield at a reduced time expenditure has been developed. The procedure described differs from the well-known method for isolation of the enzyme from the cells of Escherichia intermedia and Erwinia herbicola.(More)
116 pigmented yeast cultures were tested for the presence of L-phenylalanine-ammonia lyase transforming L-phenylalanine into trans-cinnamic acid. The enzyme was found in 54 strains. Most of these strains belonged to the genera Rhodotorula and Sporobolomyces. Toluene, along with acetone, was successfully used to increase cellular permeability of the yeast(More)
A possibility of using lactic acid as a precursor for the synthesis of L-tyrosine and L-3,4-dihydroxyphenylalanine (DOPA) by Citrobacter freundii 62 and 63 was established. The synthesis of tyrosine from lactic acid occurs at a phenol concentration of less than 0.6%. The conditions were found which enable C. freundii 62 and 63 to synthesize from lactic acid(More)
Saprophytic microorganisms belonging to different physiological groups (Azotobacter, acetic, ammonifying, lactic and nodule bacteria, a phototrophous purple bacterium of the Chromatium genus, bacteria of the Micrococcus and Pseudomonas genera, and a yeast of the Candida genus) were stored at 3-6 degrees C for 25 years in the freeze-dried state. All of the(More)
Among facultative-anaerobic bacteria utilizing formic acid, a large number of strains having tyrosine phenol lyase were found. The enzyme can catalyze synthesis of tyrosine and 3,4-dihydroxy phenyl alanine (DOPA) from pyruvate, ammonium and, accordingly, phenol and pyrocatechol. These strains were identified as Citrobacter freundii. Cell suspensions of the(More)