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By using tissue microdissection and polymerase chain reaction (PCR) techniques, we examined 85 prostate tumors that were paired with normal tissues from the same patients for allelic loss at 26 highly polymorphic microsatellite sequences, 21 spanning 8p and 5 localized to 8q. Sixty-four tumors (75%) demonstrated loss of at least one 8p locus. Separate(More)
Ammonia profoundly inhibits cAMP-dependent Cl- secretion in model T84 human intestinal crypt epithelia. Because colonic lumen concentrations of ammonia are high (10-70 mM), ammonia may be a novel regulator of secretory diarrheal responsiveness. We defined the target of ammonia action by structure-function analysis with a series of primary amines (ammonia,(More)
PURPOSE The aims of this study were to investigate telomere function in normal and Barrett's esophageal adenocarcinoma (BEAC) cells purified by laser capture microdissection and to evaluate the effect of telomerase inhibition in cancer cells in vitro and in vivo. EXPERIMENTAL DESIGN Epithelial cells were purified from surgically resected esophagi.(More)
BACKGROUND Ovarian cancer is the leading cause of mortality from gynecological malignancies, often undetectable in early stages. The difficulty of detecting the disease in its early stages and the propensity of ovarian cancer cells to develop resistance to known chemotherapeutic treatments dramatically decreases the 5-year survival rate. Chemotherapy with(More)
Two autoantibiotics produced by the fungus Candida albicans have been identified, with the aid of mass spectrometry, as 2-phenylethanol and 3-beta-hydroxyethylindole. The cyclodipeptides, cyclo(proline-leucine) and cyclo(proline-phenylalanine) were also isolated from cultures of Candida albicans, but did not inhibit the growth of the fungus.
The colon, unlike most organs, is normally exposed to high concentrations of ammonia, a weak base which exerts profound and diverse biological effects on mammalian cells. The impact of ammonia on intestinal cell function is largely unknown despite its concentration of 4-70 mM in the colonic lumen. The human intestinal epithelial cell line T84 was used to(More)
BACKGROUND Sulforaphane (SFN), an isothiocyanate phytochemical present predominantly in cruciferous vegetables such as brussels sprout and broccoli, is considered a promising chemo-preventive agent against cancer. In-vitro exposure to SFN appears to result in the induction of apoptosis and cell-cycle arrest in a variety of tumor types. However, the(More)
In response to infection by the rust pathogen Uromyces fabae the different tissues of broad bean (Vicia faba L.) showed varied pattern of amino acid metabolism; some of them being exclusively present in a particular region only. In the inflorescence tissue, for example, tryptophan, glycine, aspartic acid, serine, proline, and arginine were present. In the(More)
There were altogether fourteen amino acids in leaf sheath and culm of wheat infected with Puccinia graminis tritici, especially in and around uredial and telial pustules. Valine, tyrosine, and proline, due to their exclusive presence in uredial pustules on leaf sheath and culm of wheat, were involved in the eruption of uredospores of P. graminis tritici.(More)