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A controlled-environment membrane model for use in vitro was developed and employed in an attempt to mimic the environment of the vagina in order to study yeast-vaginal cell adhesion. Adhesion in vitro of four strains of Candida albicans (NIH 3181A, NIH 526B, ATCC 18804, and MCO 2400) to vaginal epithelial cells (VEC) appeared to be affected by the pH and(More)
Blastospores of Candida albicans, grown in YM broth (Difco, pH 6.0), were treated with tannic acid as part of a fixation schedule with glutaraldehyde and osmium tetroxide. The results obtained were compared to routine fixation procedures employing glutaraldehyde-osmium tetroxide and potassium permanganate, as well as several schedules using various(More)
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