M. A. Palmatier

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The presence of retrogradely transported endogenous nerve growth factor (NGF) in sympathetic nerves of the guinea pig was demonstrated directly by fluorescent and peroxidase immunohistochemistry in the ligated superior postganglionic nerve of the superior cervical ganglion. Fixed, frozen sections of previously ligated nerve were incubated with either rabbit(More)
Although recent animal and human experiments suggest that tissue implantation can ameliorate parkinsonism, there is controversy about what mechanism underlies recovery. Secretion of dopamine from the graft seems unlikely to be the sole restorative factor. Regenerative sprouting by the host brain may also underlie behavioral recovery. Fetal amnion and term(More)
Many laboratory procedures require the counting of cells in culture. While many cultured cells may be counted by automated methods, neuronal cultures often require manual cell counting methods that are prohibitively time-consuming. This paper examines methods of sampling from tissue culture wells for estimating total cell counts. Performance of sampling and(More)
Cells obtained from human, monkey, or rat term amnion membrane produce an activity which, in vitro, increases process outgrowth from rat sympathetic neurons and from dopaminergic neurons of the rat ventral mesencephalon. To determine if these cells could induce sprouting of dopaminergic nerve fibers in vivo, the substantia nigra of rats was lesioned(More)
Experiments were conducted to determine whether neurons in culture can serve as targets for immunologic attack mediated by major histocompatibility complex (MHC)-specific cytotoxic T lymphocytes (CTLs) which recognize Class I antigens. Allogeneic C3H/He primary neuronal cultures were quickly destroyed after CTL addition, while syngeneic C57BL/6J neurons(More)
Transplantation of dopaminergic neurons derived from fetal or adrenal tissue into the striatum is a potentially useful treatment for Parkinson's disease (PD). Although initially promising, recent clinical studies using adrenal autografts have demonstrated limited efficacy. The use of human fetal cells, despite promising preliminary results, is complicated(More)
This paper proposes a regression model for spatially correlated count data that generalizes the work of Zeger (1988, Biometrika 75, 621-629) developed in a time-series setting. In this approach, spatial correlation is introduced through a latent process, and the marginal mean function may contain spatial trends and covariates. Generalized estimating(More)
Polymer capsules were fabricated to encapsulate PC12 cells within a semipermeable and immunoprotective barrier. The inclusion of precipitated chitosan as an immobilization matrix within the polymer capsules increased the survival and physiological functioning of the PC12 cells. In an initial study, HPLC analysis revealed that the inclusion of a chitosan(More)
To determine whether the peripheral sympathetic neurons of subhuman primates are destroyed by guanacline treatment, we treated Macaca fasicularis with 2 or 20 mg/kg of guanethidine, guanacline, or the saturated analog of guanacline (SAG) 5 times per week for 4 or 12 weeks. All monkeys given 20 mg/kg of guanethidine, guanacline, or SAG showed a marked loss(More)
Cellular transplantation is rapidly becoming recognized as a possible therapeutic approach in human neurodegenerative disorders such as Parkinson’s disease (PD). The transition from basic research to broad scale clinical application requires the clear demonstration of the safety of this technology. A milestone in this transition involves the identification(More)