M A Ferrández

Learn More
The effects of T3 on cultured human fetal epiphyseal chondrocytes were assessed by studying its effects on DNA synthesis and alkaline phosphatase activity. DNA synthesis was evaluated as follows: after 48-h incubation in Ham's F-12 serum-free medium, cultured chondrocytes were incubated with or without T3 (0.1-100 nM) in MCDB-104 serum-free medium for(More)
The biological effects of dihydrotestosterone (DHT) and testosterone (T) on cultured human fetal epiphyseal chondrocytes were assessed by studying the ability of these androgens to promote DNA synthesis. DNA synthesis was evaluated by measuring [3H]thymidine incorporation into DNA. After 48-h incubation in Ham's F-12 serum-free medium, chrondrocytes were(More)
Somatostatin effects on cultured human fetal epiphyseal chondrocytes were evaluated by studying the effects of Somatostatin on DNA synthesis. Cultured epiphyseal chondrocytes from human fetuses (12–40 wk old) were incubated for 48 h in Ham's F-12 serum-free medium. After this, the medium was replaced by MCDB-104 serum-free medium and the cells were(More)
The biological activity of HPL on human fetal epiphyseal chondrocytes in culture has been investigated by studying the capacity to stimulate chondrocyte proliferation in a chemically defined serum-free medium. Chondrocytes ( 70.000 cells/well ) were incubated with HAM F-12 serum and antibiotic-free medium for 48 h. The medium was then aspirated and replaced(More)
  • 1